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T7189

Tumor Necrosis Factor Soluble Receptor II (TNF sRII)/Fc Chimera human

>95% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture

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50 μg
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$832.00

About This Item

UNSPSC Code:
51111800
NACRES:
NA.32
MDL number:
Form:
lyophilized powder
Assay:
>95% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in NSO cells
Mol wt:
52.6 kDa

$832.00


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biological source

human

Quality Segment

recombinant

expressed in NSO cells

assay

>95% (SDS-PAGE)

form

lyophilized powder

potency

0.004-0.016 mg per mL ED50

mol wt

52.6 kDa

packaging

pkg of 50 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... TNFRSF1B(7133)

Application

Tumor Necrosis Factor Soluble Receptor II (TNF sRII)/Fc Chimera human can be used as a test compound for analyzing the interaction between tumor necrosis factor (TNF) and its two soluble receptors by using surface plasmon resonance (SPR) technique.

Biochem/physiol Actions

Tumor Necrosis Factor Soluble Receptor II (TNF sRII)/Fc Chimera human belongs to TNF family of receptors and mediates the metabolic effects of TNF-α. Tumor Necrosis Factor Soluble Receptor II blocks the TNF-α-induced apoptosis and hence regulates the TNF-α function. It also has a pivotal role in neutralizing the biological activities of both TNF-α and TNF-β.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline

Analysis Note

The biological activity is measured by its ability to neutralize the TNF-α mediated cytotoxicity in mouse L-929 cells in the presence of the metabolic inhibitor actinomycin D.

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T3439T2315T3564
description

>95% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture

description

≥95% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture

description

IgG fraction of antiserum, lyophilized powder

description

>97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

assay

>95% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

-

assay

>97% (SDS-PAGE)

biological source

human

biological source

human

biological source

goat

biological source

mouse

recombinant

expressed in NSO cells

recombinant

expressed in NSO cells

recombinant

-

recombinant

expressed in E. coli

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

western blot: 1 μg/mL (detects rhs TNF RII at 5 ng/lane under non-reducing conditions), immunocytochemistry: 5-15 μg/mL (detects TNF RII in human PBLs)

technique(s)

cell culture | mammalian: suitable

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

mol wt

52.6 kDa

mol wt

48 kDa

mol wt

-

mol wt

21 kDa


Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable



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