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Tumor Necrosis Factor-β human

Name: Tumor Necrosis Factor-&#946; human
Brand: SIGMA
Price: 372.3 USD
Availability: InStock

>95% (SDS-PAGE), recombinant, expressed in E. coli, powder, suitable for cell culture | mammalian: suitable

Synonym(s):

TNF-β

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Pack Size	SKU	Availability	Price
10 μg		Check Cart for Availability	$438.00 $372.30

About This Item

UNSPSC Code:

12352202

NACRES:

NA.77

MDL number:

MFCD00163958

Biological source:

human

Recombinant:

expressed in E. coli

Assay:

>95% (SDS-PAGE)

Form:

powder

Mol wt:

protein 18.8 kDa

Impurities:

≤1 EU/μg

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Properties

Product Name

Tumor Necrosis Factor-β human, TNF-β, recombinant, expressed in E. coli, powder, suitable for cell culture

biological source

human

Quality Segment

200

recombinant

expressed in E. coli

assay

>95% (SDS-PAGE)

form

powder

potency

4-20 pg/mL Activity

quality

endotoxin tested

mol wt

protein 18.8 kDa

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤1 EU/μg

UniProt accession no.

P01374

storage temp.

−20°C

Gene Information

human ... LTA(4049), TNFSF13B(10673)

Description

Biochem/physiol Actions

Tumor necrosis factor-β (TNF-β), also known as lymphotoxin-α (LT-α) and cytotoxic factor, is a 25 kDa glycoprotein that is tightly regulated by lymphocytes. TNF-β and TNF-α share a 28% amino acid sequence identity, and as soluble forms, both can exist in solution as homotrimers, activate the same TNF receptors (TNFR1 and TNFR2) and elicit many of the same bioactivities. Unlike TNF-α, TNF-β can also form heterotrimers with one or two molecules of the related membrane-bound lymphotoxin-β (LT-β). Reportedly, LTα1/β2, the predominant heterotrimer, binds and activates only the LT-β receptor, LT-βR. The other heterotrimer, LT-α2/β1 binds and activates TNFR1 and TNFR2 but not LT-βR. TNF-β is expressed in activated T, B and NK cells and in certain transformed cells. Genes for TNF-β, LT-β and TNF-α are tightly linked near each other within the MHC. TNF-β appears to play many of the same biological roles as TNF-α, but the membrane-bound form with LT-β is critical for normal lymphoid organ development. Genetic polymorphisms in TNF-α and TNF-β have been linked to certain pathological conditions, including myasthenia gravis. At the amino acid level, human and mouse TNF-β are 74% homologous and human and mouse LT-β are 80% homologous.

Tumor necrosis factor-β, also known as lymphotoxin-α (LT-α) and cytotoxic factor, is a 25 kDa glycoprotein that is tightly regulated by lymphocytes.

Physical form

lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein

Analysis Note

The biological activity is measured in a cytotoxicity assay using the TNF-suspectible murine L929 cell line in the presence of actinomycin D.

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This Item	T7539	T5944	T2815
Sigma-Aldrich T7799 Tumor Necrosis Factor-β human	Sigma-Aldrich T7539 Tumor Necrosis Factor-α from mouse	Sigma-Aldrich T5944 Tumor Necrosis Factor-α from rat	Sigma-Aldrich T2815 Transforming Growth Factor-β2 human
assay >95% (SDS-PAGE)	assay ≥95%	assay ≥98% (SDS-PAGE and HPLC)	assay ≥97% (electrophoresis)
recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in NSO cells
biological source human	biological source mouse	biological source rat	biological source human
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
form powder	form powder	form powder	form powder

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Global Trade Item Number

SKU	GTIN
T7799-10UG	04061837378645

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