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T7915

Thioredoxin Reductase from Escherichia coli

ammonium sulfate suspension, >25 units/mg protein (Bradford)

Synonym(s):

NADPH:oxidized thioredoxin oxidoreductase

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250 μg

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$835.00

About This Item

CAS Number:
9074-14-0
UNSPSC Code:
12352204
NACRES:
NA.32
EC Number:
1.8.1.9 (BRENDA, IUBMB)
MDL number:
MFCD00678149
Specific activity:
>25 units/mg protein (Bradford)
Biological source:
Escherichia coli

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biological source

Escherichia coli

Quality Segment

form

ammonium sulfate suspension

specific activity

>25 units/mg protein (Bradford)

mol wt

70 kDa

technique(s)

activity assay: suitable

UniProt accession no.

storage temp.

2-8°C

Gene Information

Escherichia coli K12 ... trxB(949054)

General description

Research area: Cell Signaling
Thioredoxin reductases (TrxRs) belongs to family of selenium-containing pyridine nucleotide-disulphide oxidoreductases.

Application

Thioredoxin Reductase from Escherichia coli can be used in peroxidase-coupled thioredoxin system assay for assessing the peroxidase activitiy of Cys-based thiol peroxidases.Thioredoxin Reductase from Escherichia coli has been used:
  • for determining the enzymatic activity of His6-Ahp1p.
  • to assay C. elegans TRXR using the TXN-dependent activity assay.
  • to investigate the biological reducing systems for organic hydroperoxide resistance R gene (OhrR).
  • for enzymatic targeting of auranofin to test its antimicrobial properties.
  • in thioredoxin reductase activity and peroxiredoxin assay.
  • to study the synergy between broccoli sprout extract and selenium in the upregulation of thioredoxin reductase in human hepatocytes.
Thioredoxin reductase from Escherichia coli has been used in thioredoxin reductase activity and peroxiredoxin assay.
It has also been used to study the synergy between broccoli sprout extract and selenium in the upregulation of thioredoxin reductase in human hepatocytes.

Biochem/physiol Actions

Thioredoxin reductase is a FAD containing enzyme, which transfers the reducing equivalent from NADPH to the disulphide bond of the enzyme by using FAD moiety within the Cys-Ala-Thr-Cys sequence. It can also reduce Trx-S2 to Trx-(SH)2 by using NADPH.
An FAD-containing enzyme involved in the transfer of hydrogen from E. coli thioredoxin to other proteins thus providing a powerful disulfide reductase system.
Thioredoxin reductase (TrxR) is an NADPH-dependent oxidoreductase containing one FAD per subunit that reduces the active site disulfide in oxidised thioredoxin (Trx). The molecular weight of the isozymes from mammalian sources vary between 55-67 kDa as compared with 35 kDa in prokaryotes, plants or yeast. The substrate specificity of the mammalian enzyme is much broader than the prokaryotic enzyme reducing both mammalian and E. coli thioredoxins as well as well as non-disulfide substrates such selenite, lipoic acids, lipid hydroperoxides and hydrogen peroxide.

Physical form

Suspension in 3.6 M (NH4)2SO4 containing 30 mM potassium phosphate buffer, pH 7.5, and 2 mM EDTA.

Other Notes

One unit will cause an increase in absorbance of 1.0 at 412 nm (when measured in a coupled assay with E. coli thioredoxin and DTNB) per min per mL at pH 7.0 at 25 °C.

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Thioredoxin Reductase from Escherichia coli ammonium sulfate suspension, >25 units/mg protein (Bradford)

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Sigma-Aldrich

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Thioredoxin from Escherichia coli

Phosphatase, Alkaline from Escherichia coli ammonium sulfate suspension, 30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)

Sigma-Aldrich

P4252

Phosphatase, Alkaline from Escherichia coli

β-Galactose Dehydrogenase from Pseudomonas fluorescens recombinant, expressed in E. coli, ammonium sulfate suspension, ≥50 units/mg protein (biuret)

Sigma-Aldrich

G6637

β-Galactose Dehydrogenase from Pseudomonas fluorescens

description

ammonium sulfate suspension, >25 units/mg protein (Bradford)

description

recombinant, expressed in E. coli, essentially salt-free, lyophilized powder, ≥3 units/mg protein

description

ammonium sulfate suspension, 30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)

description

recombinant, expressed in E. coli, ammonium sulfate suspension, ≥50 units/mg protein (biuret)

specific activity

>25 units/mg protein (Bradford)

specific activity

≥3 units/mg protein

specific activity

30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)

specific activity

≥50 units/mg protein (biuret)

biological source

Escherichia coli

biological source

Escherichia coli

biological source

Escherichia coli

biological source

Pseudomonas fluorescens

Gene Information

Escherichia coli K12 ... trxB(949054)

Gene Information

Escherichia coli K12 ... trxA(948289)

Gene Information

-

Gene Information

Pseudomonas fluorescens ... gdh(533113295)

technique(s)

activity assay: suitable

technique(s)

activity assay: suitable

technique(s)

-

technique(s)

-

form

ammonium sulfate suspension

form

essentially salt-free, lyophilized powder

form

ammonium sulfate suspension

form

ammonium sulfate suspension

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

Storage Class

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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