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U1500

Urease from Canavalia ensiformis (Jack bean)

Type III, powder, 15,000-50,000 units/g solid

Synonym(s):

Jack bean urease, Urea amidohydrolase

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Size/SKUAvailabilityPrice
20000 units

Available to ship TODAYfromMILWAUKEE

$94.30
100000 units

Available to ship TODAYfromMILWAUKEE

$336.00
500000 units

Available to ship TODAYfromMILWAUKEE

$1,190.00
1000000 units

Available to ship TODAYfromMILWAUKEE

$1,910.00

About This Item

CAS Number:
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-656-0
NACRES:
NA.54
MDL number:
EC Number:
Specific activity:
15,000-50,000 units/g solid
Biological source:
Canavalia ensiformis

$94.30


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biological source

Canavalia ensiformis

Quality Segment

type

Type III

form

powder

specific activity

15,000-50,000 units/g solid

mol wt

~544620 Da

application(s)

diagnostic assay manufacturing

storage temp.

2-8°C

General description

Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620
Urease is a cytosolic enzyme that catalyzes the hydrolysis of urea to ammonia and carbon dioxide, with initial conversion to carbamate, and the carbamate breaks down further, on hydrolysis, into ammonia and to carbonic acid / carbonate. Urease is a metalloenzyme that contains nickel as a co-factor. Urease occurs naturally in plants, fungi, and certain bacteria. James B. Sumner first isolated urease from jack bean in 1926.

Biochem/physiol Actions

Urease from Canavalia ensiformis functions as an insecticide by the release of a toxic small peptide cleaved by proteases found in the gut of insects.

Other Notes

One micromolar unit will liberate 1.0 μmole of NH3 from urea per min at pH 7.0 at 25 °C. It is equivalent to 1.0 I.U. or 0.054 Sumner unit (1.0 mg ammonia nitrogen in 5 minutes at pH 7.0 at 20 °C)

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This Item
94280A2264C2272
description

Type III, powder, 15,000-50,000 units/g solid

description

powder, ~1 U/mg

description

ammonium sulfate suspension, ≥8 units/mg protein

description

biotin conjugate, Type IV, lyophilized powder

biological source

Canavalia ensiformis

biological source

-

biological source

Canavalia ensiformis

biological source

Canavalia ensiformis

specific activity

15,000-50,000 units/g solid

specific activity

~1 U/mg

specific activity

≥8 units/mg protein

specific activity

-

form

powder

form

powder

form

ammonium sulfate suspension

form

lyophilized powder

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

-

application(s)

-

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

mol wt

~544620 Da

mol wt

Mr ~480000

mol wt

-

mol wt

-


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pictograms

Health hazardExclamation mark

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 1



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Questions

1–3 of 3 Questions  
  1. what's the minimum quantity to purchase?

    1 answer
    1. The smallest package size available for this item is 20,000 units. The activity varies from lot to lot and may range from 15,000 - 50,000 units per gram. The 20,000 unit package size may contain 0.400 - 1.33 grams of powder.

      Helpful?

  2. Do you have Food Grade / Pharma Grade Powder for the same

    1 answer
    1. Unfortunately, a food grade or pharma grade Urease powder is not currently available.

      Helpful?

  3. WHAT IS THE ENZYME ACTIVITY

    1 answer
    1. The lot-specific activity is reported in the Certificate of Analysis.

      Please see the link below to access a specific lot or review a sample Certificate:
      https://www.sigmaaldrich.com/product/sigma/U1500#product-documentation

      Helpful?

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