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U9382

Ubiquitin-Carrier Protein H10 human

≥95% (SDS-PAGE), recombinant, expressed in E. coli (histidine-tagged), buffered aqueous glycerol solution

Synonym(s):

UbcH10

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100 μg
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$804.00

About This Item

UNSPSC Code:
12352202
NACRES:
NA.32
MDL number:
Form:
buffered aqueous glycerol solution
Assay:
≥95% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in E. coli (histidine-tagged)
Mol wt:
~20 kDa

$804.00


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biological source

human

Quality Segment

recombinant

expressed in E. coli (histidine-tagged)

assay

≥95% (SDS-PAGE)

form

buffered aqueous glycerol solution

mol wt

~20 kDa

technique(s)

ligand binding assay: suitable

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... UBE2C(11065)

General description

Manufactured for Sigma by Boston Biochem., Inc.

Application

Ubiquitin-Carrier Protein H10 (UbcH10) human may be used to study the role of ubiquitin-proteasome system in pathogenesis of human diseases.

Biochem/physiol Actions

Ubiquitin-Carrier Protein H10 human also referred to as UbcH10 is a recombinant protein that catalyzes the destruction of cyclin A as well as cyclin B along with the anaphase promoting complex and hence plays a pivotal role in controlling the cell exit from mitosis.
In conjunction with anaphase-promoting complex, catalyzes both cyclin A and cyclin B destruction.
In conjunction with the anaphase-promoting complex, the protein catalyzes the destruction of both cyclin A and cyclin B.

Physical form

Solution in 50 mM HEPES, pH 8.0, 125 mM NaCl, and 1 mM DTT in 10% glycerol.

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≥95% (SDS-PAGE), recombinant, expressed in E. coli (histidine-tagged), buffered aqueous glycerol solution

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description

≥95% (SDS-PAGE), recombinant, expressed in E. coli, liquid

technique(s)

ligand binding assay: suitable

technique(s)

immunohistochemistry: 1:500- 1:1000

technique(s)

immunoblotting: 0.04-0.4 μg/mL, immunofluorescence: 0.25-2 μg/mL

technique(s)

-

biological source

human

biological source

rabbit

biological source

rabbit

biological source

-

assay

≥95% (SDS-PAGE)

assay

-

assay

-

assay

≥95% (SDS-PAGE)

recombinant

expressed in E. coli (histidine-tagged)

recombinant

-

recombinant

-

recombinant

expressed in E. coli

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

liquid

mol wt

~20 kDa

mol wt

-

mol wt

-

mol wt

18 kDa


WGK

WGK 1

Storage Class

10 - Combustible liquids

Flash Point (°F)

235.4 °F - closed cup

Flash Point (°C)

113 °C - closed cup



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