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V1385

VEGF Receptor-1 (Flt-1)/Fc Chimera human

>90% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder

Synonyme(s) :

Vascular Endothelial Growth Factor Receptor‑1

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À vous/RéférenceDisponibilitéPrix
50 μg
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763,00 €

A propos de cet article

UNSPSC Code:
51111800
NACRES:
NA.32
MDL number:
MFCD01323295
Biological source:
human
Recombinant:
expressed in baculovirus infected Sf21 cells
Assay:
>90% (SDS-PAGE)
Form:
lyophilized powder
Mol wt:
predicted mol wt ~100 kDa
Impurities:
endotoxin, tested

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biological source

human

Quality Segment

100

recombinant

expressed in baculovirus infected Sf21 cells

assay

>90% (SDS-PAGE)

form

lyophilized powder

potency

≤35 ng/mL ED50

mol wt

predicted mol wt ~100 kDa

packaging

pkg of 50 μg

storage condition

avoid repeated freeze/thaw cycles

impurities

endotoxin, tested

UniProt accession no.

P17948

storage temp.

−20°C

Gene Information

human ... FLT1(2321)

Application

VEGF Receptor-1 (Flt-1)/Fc chimera human may be used tostudy vasculogenesis and angiogenesis.

Biochem/physiol Actions

VEGF Receptor-1 (VEGF R1) also known as Flt-1 belongs to class III subfamily of RTKs and is a potent VEGF antagonist expressed mainly in endothelial cells. VEGF R1 binds to vascular endothelial growth factor B (VEGF-B) and regulates the activity of plasminogen activator in endothelial cells. It also assists endothelial cells in the proper spatial organization of lumen-containing vessels. Additionally, alternatively spliced VEGF R1 pre-mRNA helps in regulating the VEGF activity in angiogenesis. VEGF R1 also activates PLC gamma which in turn produces cellular signaling molecules diacylglycerol as well as inositol 1,4,5-trisphosphate and activates protein kinase C.
VEGF R1 binds VEGF with high affinity and is a potent VEGF inhibitor.
VEGF receptors, class III receptor tyrosine kinases, have seven Ig like extracellular motifs and a tyrosine kinase intracellular domain split by a kinase insert sequence. VEGFR 1 was originally called fms like tyrosine kinase 1 (flt 1), indicating its homology to receptors of PDGF, M CSF, and SCF. VEGFR 1 is one of the five receptor tyrosine kinases (KDR/Flk-1, Flt-4, tie-1 and tek/tie-2) whose expression is almost exclusively restricted to endothelial cells. These receptors are likely to play central roles in vasculogenesis and angiogenesis. Recombinant VEGF R1 binds VEGF with high affinity and is a potent VEGF inhibitor. VEGFR 1 and 2 are both expressed in an endothelial cell-specific manner and are detectable in virtually all tissues in adults and embryos. Hypoxia induces endothelial cell expression of VEGFR 1 but not VEGFR 2. Monocytes express VEGFR 1 and 2 and can migrate to the site of VEGF release, but this response appears to be VEGFR 1 mediated. VEGFR 2 is also expressed in pancreatic duct cells. VEGFR 2 is involved in commitment of endothelial-cell lineages and to cell proliferation, while VEGFR 1 seems to be responsible for guiding endothelial cells into the proper spatial organization into lumen-containing vessels. VEGFR 2 is a key marker for pluripotent HSCs. Neurophilins, a group of unrelated receptors for some VEGF family members may also act as coreceptors to facilitate VEGF binding to VEGFR 2. VEGFR 3 is a specific marker for lymphatic vessels. VEGFR 3 has also been detected on some high endothelial venules, in embryonic pre-lymphatic blood vessels, some tumor blood vessels, and certain hematopoietic and leukemia cells.

Physical form

Lyophilized from a 0.2 μm filtered solution in 20 mM MOPS, 500 mM sodium chloride, 0.05% CHAPS, pH 7.0 containing 50 μg bovine serum albumin per 1 μg of cytokine

Analysis Note

The biological activity is measured by its ability to Inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells.

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Cet article
V6758E4643SRP0348
VEGF Receptor-1 (Flt-1)/Fc Chimera human >90% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder

Sigma-Aldrich

V1385

VEGF Receptor-1 (Flt-1)/Fc Chimera human

VEGF Receptor-2 (Flk-1, KDR)/Fc Chimera human >90% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder

Sigma-Aldrich

V6758

VEGF Receptor-2 (Flk-1, KDR)/Fc Chimera human

Heparin-Binding EGF-Like Growth Factor human HB-EGF, recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture

Sigma-Aldrich

E4643

Heparin-Binding EGF-Like Growth Factor human

Flt1 active human recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE)

Sigma-Aldrich

SRP0348

Flt1 active human

description

>90% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder

description

>90% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder

description

HB-EGF, recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture

description

recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE)

biological source

human

biological source

human

biological source

human

biological source

human

assay

>90% (SDS-PAGE)

assay

>90% (SDS-PAGE)

assay

≥97% (SDS-PAGE and N-terminal analysis)

assay

≥70% (SDS-PAGE)

recombinant

expressed in baculovirus infected Sf21 cells

recombinant

expressed in NSO cells

recombinant

expressed in baculovirus infected Sf21 cells

recombinant

expressed in baculovirus infected Sf9 cells

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

-

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

aqueous solution

UniProt accession no.

P17948

UniProt accession no.

P35968

UniProt accession no.

Q99075

UniProt accession no.

P17948

Classe de stockage

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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