V4014
Anti-V5-Cy3™ antibody, Mouse monoclonal
purified immunoglobulin, ~1 mg/mL, clone V5-10
Synonym(s):
Monoclonal Anti-V5
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 100 μg | Please contact Customer Service for Availability | $651.00 |
About This Item
Conjugate:
CY3 conjugate
Clone:
V5-10, monoclonal
Application:
ICC
Citations:
6
$651.00
Please contact Customer Service for Availability
biological source
mouse
Quality Segment
conjugate
CY3 conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
V5-10, monoclonal
concentration
~1 mg/mL
technique(s)
immunocytochemistry: 2.0-4.0 μg/mL
shipped in
wet ice
storage temp.
2-8°C
General description
Monoclonal Anti-V5 (mouse IgG1 isotype) is derived from the V5 hybridoma, produced by fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino acid residues GKPIPNPLLGLDST (95-108) of the P/V proteins of the Paramyxovirus SV5, conjugated to KLH. The conjugate is then extensively dialyzed to remove unbound Cy3 fluorophore. Monoclonal Anti-V5, Cy3 conjugate recognizes the V5 tag sequence on V5 tagged fusion proteins by immunocytochemistry.
Application
Many recombinant proteins have been engineered to express a short sequence derived from the V5 molecule known as V5-tag.[1][2] This tag facilitates the detection, isolation and purification of the proteins.[3] Monoclonal antibodies reacting specifically with V5 and conjugated to Cy3 may be useful in various immunotechniques, and also to identify the expression of a V5 tagged fusion protein in situ by immunofluorescence.
Biochem/physiol Actions
Recognizes V5 Tag (GKPIPNPLLGLDST) fusion proteins
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Legal Information
Cy3 is a trademark of Cytiva
1 of 1
This Item | |||
|---|---|---|---|
| antibody form purified immunoglobulin | antibody form purified from hybridoma cell culture | antibody form purified immunoglobulin | antibody form purified from hybridoma cell culture |
| clone V5-10, monoclonal | clone V5-10, monoclonal | clone V5-10, monoclonal | clone CY-96, monoclonal |
| conjugate CY3 conjugate | conjugate unconjugated | conjugate peroxidase conjugate | conjugate unconjugated |
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
| shipped in wet ice | shipped in dry ice | shipped in - | shipped in dry ice |
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Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Related Content
Global Trade Item Number
| SKU | GTIN |
|---|---|
| V4014-100UG | 04061838217455 |