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V4388

Anti-VP16 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Anti Alpha trans-inducing protein

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200 μL
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$784.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.56
MDL number:
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IP, WB
Citations:
17

$784.00


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biological source

rabbit

Quality Segment

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

immunoprecipitation (IP): 10 μg using mammalian cell extracts expressing VP16 fusion proteins, western blot: 1:1,000 using mammalian cell extracts expressing VP16 fusion proteins

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Anti-VP16 is produced in rabbit using a synthetic peptide corresponding to amino acids 474-487 of the herpes simplex virus VP16 protein, conjugated to KLH via an N-terminal added cysteine residue. Whole antiserum is fractionated and further purified by ionexchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.

Immunogen

synthetic peptide corresponding to amino acids 474-487 of the herpes simplex virus VP16 protein, conjugated to KLH via an N-terminal added cysteine residue.

Application

Anti-VP16 recognizes VP16 fusion proteins by immunoblotting and immunoprecipitation. It is used to study the effect of sialic acid on herpes simplex virus type 1 envelope glycoproteins. It is also used to study if self-association of lymphocytic choriomeningitis virus nucleoprotein is mediated by its N-terminal region and is not required for its anti-interferon function.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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This Item
R3779S5188SAB4200119
antibody form

IgG fraction of antiserum

antibody form

IgG fraction of antiserum

antibody form

IgG fraction of antiserum

antibody form

purified immunoglobulin

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

peroxidase conjugate

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rat

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

6F7, monoclonal

technique(s)

immunoprecipitation (IP): 10 μg using mammalian cell extracts expressing VP16 fusion proteins, western blot: 1:1,000 using mammalian cell extracts expressing VP16 fusion proteins

technique(s)

indirect immunofluorescence: 1:500-1:1,000 using human HeLa cells fixed with paraformaldehyde-Triton, microarray: suitable, western blot: 1:2,000-1:4,000 using MCF7 nuclear extracts

technique(s)

indirect immunofluorescence: 1:2,000 using 3% paraformaldehyde-fixed, 0.5% Triton X-100 treated human epidermal carcinoma A431cell line, microarray: suitable, western blot: 1:2,000 using whole cell extract of the human epidermal carcinoma A431 cell line

technique(s)

western blot: 1:1,000-1:2,000 using extracts of transfected cells expressing C-terminal FLAG-tagged fusion protein


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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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Global Trade Item Number

SKUGTIN
V4388-200UL04061838222916

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