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Anti-phospho-Vinculin (pTyr⁸²²) antibody produced in rabbit

Name: Anti-phospho-Vinculin (pTyr<SUP>822</SUP>) antibody produced in rabbit
Brand: SIGMA

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-CMD1W, Anti-CMH15, Anti-HEL114, Anti-MV, Anti-MVCL

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD06798505

Conjugate:

unconjugated

Clone:

polyclonal

Application:

Citations:

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

human, chicken, rat, mouse

technique(s)

western blot: 1:1,000 using chick embryo fibroblasts (CEFs) transfected with activated Src.

UniProt accession no.

P18206

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pTyr822)

Gene Information

human ... VCL(7414)
mouse ... Vcl(22330)
rat ... Vcl(305679)

Description

General description

Vinculin (VCL) is a ubiquitously expressed cytoskeletal protein, encoded by the gene mapped to human chromosome 10q11.2-qter. VCL is a vital element of adherens type cell junctions. Vinculin structure contains a globular head connected to a tail domain by a short proline-rich sequence, and an intramolecular interaction between the head and tail hides the various ligand-binding sites in the protein.

Immunogen

synthetic phosphopeptide derived from the region of vinculin that contains tyrosine 822.

Application

Anti-phospho-Vinculin (pTyr822) antibody produced in rabbit has been used in immunofluorescence staining.

Biochem/physiol Actions

Vinculin (VCL) is a cytoskeletal protein that supports cell-to-cell and cell to matrix junctions. It is also essentially involved in normal embryonic development and cardiac function, due to its role in controlling adhesion and motility, as well as cell spreading.In mature focal adhesions (FAs), VCL acts as an important constituent of the “molecular clutch” that induces the transmission of force from cytoplasmic F-actin to membrane-bound integrins. Aberration or mutation of VCL leads to cardiomyopathy, which is associated with hypertension. Experimental studies reveal that, VCL might be used as a biomarker of aging or heart failure. Vinculin associates with FAs and assists in several cellular functions, such as stabilizing and strengthening FAs and promoting their maturation, extracellular matrix (ECM) mechanosensing, regulating actin cytoskeletal dynamics and signaling pathways involved in apoptosis.

Physical form

Solution in Dulbecco′s phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.3, with 50% glycerol, 1.0 mg/mL BSA (IgG and protease free) and 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	V4139	SAB4200080	V4505
Sigma-Aldrich V4889 Anti-phospho-Vinculin (pTyr⁸²²) antibody produced in rabbit	Sigma-Aldrich V4139 Anti-Vinculin antibody produced in rabbit	Sigma-Aldrich SAB4200080 Anti-Vinculin antibody, Mouse monoclonal	Sigma-Aldrich V4505 Monoclonal Anti-Vinculin antibody produced in mouse
description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody, buffered aqueous solution	description clone V284, purified from hybridoma cell culture	description clone VIN-11-5, ascites fluid
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form purified from hybridoma cell culture, purified immunoglobulin	antibody form ascites fluid
biological source rabbit	biological source rabbit	biological source mouse	biological source mouse
Gene Information human ... VCL(7414) mouse ... Vcl(22330) rat ... Vcl(305679)	Gene Information human ... VCL(7414) mouse ... Vcl(22330) rat ... Vcl(305679)	Gene Information human ... VCL(7414) mouse ... Vcl(22330) rat ... Vcl(305679)	Gene Information human ... VCL(7414) mouse ... Vcl(22330)
species reactivity human, chicken, rat, mouse	species reactivity rat, human, mouse	species reactivity rabbit, chicken, human, rat, monkey, mouse, canine	species reactivity bovine, human, chicken, mouse

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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