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Anti-WRN antibody, Mouse monoclonal

Name: Anti-WRN antibody, Mouse monoclonal
Brand: SIGMA
Price: 600.1 USD
Availability: InStock

clone 195C, purified from hybridoma cell culture

Synonym(s):

Anti-RECQ3, Anti-RECQL2, Anti-RECQL3, Anti-Werner syndrome helicase, Anti-Werner syndrome protein

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Size/SKU	Availability	Price
200 μL	Available to ship TODAYfromMILWAUKEE	$706.00 $600.10

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD02264220

Conjugate:

unconjugated

Clone:

195C, monoclonal

Application:

ELISA (i), IHC, IP, WB

Citations:

$600.10

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

195C, monoclonal

form

buffered aqueous solution

mol wt

antigen ~167 kDa

species reactivity

human

concentration

~1 mg/mL

technique(s)

immunohistochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, western blot: 2-4 μg/mL using nuclear cell extract of HeLa cells

isotype

IgG1

UniProt accession no.

Q14191

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... WRN(7486)

Description

General description

Anti-WRN antibody, Mouse monoclonal, (mouse IgG1 isotype) is derived from the hybridoma 195C produced by the fusion of mouse myeloma cells (p3-NS1/Ag4-1) and splenocytes from BALB/c mice immunized with a recombinant fusion protein fragment of human WRN. Werner syndrome ATP-dependent helicase (WRN) is located on chromosome 8 and is 167 kDa. The WRN protein belongs to the RecQ helicase family of DNA helicases.

Immunogen

recombinant fusion protein fragment of human WRN (amino acids 1074-1432).

Application

Anti-WRN antibody has been used in

enzyme linked immunosorbent assay (ELISA)
immunoblotting
immunoprecipitation
immunohistochemistry
western blotting

Biochem/physiol Actions

Werner syndrome ATP-dependent helicase (WRN) protein has been demonstrated to possess three known catalytic activities: 3′-5′ helicase, exonuclease and ATPase activities. The C-terminal region of the protein contains a nuclear localization signal (NLS), thus targeting WRN protein to the nucleus, nucleolus and nucleoplasmic foci. The involvement of WRN in multiple DNA metabolic process suggests its function as a tumor suppressor. Mutations in the WRN protein results in Werner syndrome (WS). The epigenetic inactivation of WRN leads to loss of WRN-exonuclease activity, resulting in increased chromosomal instability and hypersensitivity to chemotherapeutic drugs.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	SAB1404529	WH0007486M9	SAB4504327
Sigma-Aldrich W0393 Anti-WRN antibody, Mouse monoclonal	Sigma-Aldrich SAB1404529 Monoclonal Anti-WRN, (C-terminal) antibody produced in mouse	Sigma-Aldrich WH0007486M9 Monoclonal Anti-WRN antibody produced in mouse	Sigma-Aldrich SAB4504327 Anti-phospho-Werner Syndrome Helicase (pSer¹¹⁴¹) antibody produced in rabbit
description clone 195C, purified from hybridoma cell culture	description clone 2F7, purified immunoglobulin, buffered aqueous solution	description clone 3C11, purified immunoglobulin, buffered aqueous solution	description affinity isolated antibody
clone 195C, monoclonal	clone 2F7, monoclonal	clone 3C11, monoclonal	clone polyclonal
Gene Information human ... WRN(7486)	Gene Information human ... WRN(7486)	Gene Information human ... WRN(7486)	Gene Information human ... WRN(7486)
biological source mouse	biological source mouse	biological source mouse	biological source rabbit
antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form affinity isolated antibody
species reactivity human	species reactivity human	species reactivity rat, mouse, human	species reactivity human, rat, mouse
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

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