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W4387

Wee1, active from rat

N-terminal GST-tagged, solution

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60 units
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$975.00

About This Item

UNSPSC Code:
12352202
NACRES:
NA.77
MDL number:
MFCD03459055
Biological source:
rat
Recombinant:
expressed in E. coli

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biological source

rat

Quality Segment

recombinant

expressed in E. coli

form

solution

mol wt

~110 kDa

UniProt accession no.

application(s)

cell analysis

shipped in

dry ice

storage temp.

−70°C

Gene Information

rat ... Wee1(308937)

Application

Wee1, active from rat may be used to study apoptotic signaling.

Biochem/physiol Actions

Wee1 is a protein tyrosine kinase that phosphorylates Cdc2 on Tyr15 and hence inactivates the CDK1 and CDK2, which facilitates in the negative regulation of entry of cells into mitosis. Increased level of wee1 stimulates Human Immunodeficiency Virus type 1 (HIV-1) Vpr induced cell cycle arrest at G2/M transition followed by apoptosis.
Protein inhibitor of Cdk2 kinase

Physical form

Solution in 50 mM Tris-HCl, pH 7.5, 1 mM EDTA, 1 mM DTT, 10% Glycerol

Other Notes

One unit of Wee 1 is defined as the amount of Wee 1 which inhibits 90% of the activity of 10 units of Cdk2

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Wee1, active from rat N-terminal GST-tagged, solution

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PIP5K1C, active, GST tagged human

description

N-terminal GST-tagged, solution

description

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

description

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

description

PRECISIO®, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Gene Information

rat ... Wee1(308937)

Gene Information

human ... PDK1(5163)

Gene Information

human ... PDK2(5164)

Gene Information

human ... PIP5K1C(23396)

biological source

rat

biological source

human

biological source

human

biological source

human

application(s)

cell analysis

application(s)

cell analysis

application(s)

cell analysis

application(s)

cell analysis

recombinant

expressed in E. coli

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in baculovirus infected Sf9 cells

form

solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

UniProt accession no.

Q63802

UniProt accession no.

-

UniProt accession no.

-

UniProt accession no.

-

Storage Class

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
071K0243

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