Anti-CIROP Antibody, clone 3G14 ZooMAb^® Rabbit Monoclonal

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.
Ciliated left-right organizer metallopeptidase (UniProt: A0A1B0GTW7; also known as EC:3.4.24.-, Leishmanolysin-like peptidase 2) is encoded by the CIROP (also known as LMLN2) gene (Gene ID: 100128908) in human. The ciliated left-right organizer metallopeptidase (CIROP) is a zinc-dependent metalloprotease crucial for establishing left-right asymmetry during vertebrate embryonic development. Encoded by the CIROP gene, this protein features a signal peptide, a zinc catalytic domain with a consensus active site motif (His-Glu-x-x-His), a cysteine-rich domain, and a C-terminal transmembrane domain. CIROP is specifically expressed in ciliated left-right organizers (LROs), such as the node in mice and Kupffer′s vesicle in zebrafish, where it is essential for proper function of motile cilia. These cilia generate directional fluid flow that triggers asymmetric activation of the Nodal pathway, a signaling cascade responsible for the left-right organization of internal organs. In terms of subcellular localization, CIROP is membrane-associated, with its catalytic domain exposed extracellularly. It interacts with other proteins and substrates localized in the LRO to regulate signaling and structural components of the cilia. Dysregulation of CIROP or mutations in its gene are implicated in heterotaxy syndrome (HTX12), an autosomal recessive disorder characterized by randomization of organ placement along the left-right axis, often accompanied by severe congenital malformations, especially in the cardiovascular system.  This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

KLH-conjugated linear peptide corresponding to 26 amino acids from the N-terminal half of human CIROP.

Quality Control Testing
Evaluated by Western Blotting in CIROP-transfected HEK-293T cell lysate. 
Western Blotting Analysis: A 1:40,000 dilution of this antibody detected CIROP in CIROP-transfected HEK-293T cell lysate.
Tested Applications
Western Blotting Analysis: A 1:40,000 dilution from a representative lot detected CIROP in CIROP-transfected HEK-293T cell lysate, but not in untransfected HEK-293T cell lysate.
Affinity Binding Assay: A representative lot of this antibody bound with CIROP peptide with at least one million-fold (1,000,000X) higher affinity than with non-specific control peptide.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected CIROP in HEK-293T cells transfected with CIROP-Strep-Tag.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 3G14 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects CIROP. It targets an epitope within 26 amino acids from the N-terminal half.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.