A3010

Acylase I aus Schweineniere

Grade I, lyophilized powder, ≥1500 units/mg protein

• Synonym(e): Aminoacylase, N-Acylaminosäure-Amidohydrolase
• CAS-Nummer: 9012-37-7
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-732-3
• MDL number: MFCD00081285
• EG-Nummer: 3.5.1.14 (BRENDA, IUBMB)
• Specific activity: ≥1500 units/mg protein

Eigenschaften

• type: Grade I
• Quality Segment: 200
• form: lyophilized powder
• specific activity: ≥1500 units/mg protein
• composition: Protein, ≥60%
• UniProt accession no.: P37111
• storage temp.: −20°C
• Gene Information: pig ... ACY1(396930)

Beschreibung

Application

Acylase I from porcine kidney has been used to study the acylase I-catalyzed deacetylation of various S-alkyl-N-acetyl-L-cysteines and their carbon and oxygen analogues . Acylase I may be useful to catalyze N-acetyl amino acids to enantiomerically pure L-amino acids .

Biochem/physiol Actions

Acylase I catalyzes the deacetylation of N-acetyl-L-cysteine and S-alkyl-N-acetyl-L-cysteines. n-Butylmalonic acid is an inhibitor of acylase I. S-alkyl-N-acetyl-L-cysteines with short (C0-C3) and unbranched S-alkyl substituents have been found to be good acylase I substrates .

Analysis Note

Proteingehalt mittels Biuret-Reaktion bestimmt.

Other Notes

1 U hydrolysiert 1.0 μmol N-Acetyl-L-methionin pru Stunde bei pH 7.0 und 25 °C.

Sicherheitshinweise

• Piktogramme: GHS08,GHS07
• Signalwort: Danger
• Gefahrencodes: H315,H319,H334,H335
• Sicherheitshinweise: P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Zielorgane: Respiratory system
• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• PSA (Persönliche Schutzausrüstung): Eyeshields, Gloves, type N95 (US)