Protease Inhibitor Cocktail

for use in tissue culture media, DMSO solution

EC Number:
MDL number:

Quality Level


DMSO solution

shipped in

dry ice

storage temp.


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Inhibits serine, cysteine, aspartic proteases, and aminopeptidases


1 mL in glass bottle


After 48 hours in medium, add fresh inhibitors in fresh medium.


Pepstatin A

Other Notes

Mixture of protease inhibitors with broad specificity for the inhibtion of serine, cysteine, aspartic and aminopeptidases. Contains aprotinin, bestatin, leupeptin, E-64, and pepstatin A. Contains no metal chelators


After 48 hours exposure, the product is non-toxic to adherent cell lines A431, CHO, COS, HepG2 and HeLa; and to Jurkat and HL-60 cell lines grown in suspension.


Use at a dilution of 1:200 or more in tissue culture media to prevent proteolytic degradation of secreted proteins.

Physical form

Solution in DMSO (D 2650, Hybri-Max).


NA 1993 / PGIII

WGK Germany


Flash Point(F)

188.6 °F

Flash Point(C)

87 °C

Certificate of Analysis
Certificate of Origin
Matthew Townsend et al.
The Journal of physiology, 572(Pt 2), 477-492 (2006-02-14)
The accumulation of amyloid beta-protein (Abeta) in brain regions serving memory and cognition is a central pathogenic feature of Alzheimer's disease (AD). We have shown that small soluble oligomers of human Abeta that are naturally secreted by cultured cells inhibit...
Takuma Shiratori et al.
Journal of immunology (Baltimore, Md. : 1950), 200(1), 218-228 (2017-11-17)
As osteoclasts have the central roles in normal bone remodeling, it is ideal to regulate only the osteoclasts performing pathological bone destruction without affecting normal osteoclasts. Based on a hypothesis that pathological osteoclasts form under the pathological microenvironment of the...
Yu Li et al.
Animals : an open access journal from MDPI, 10(4) (2020-04-02)
Dairy cows usually experience negative energy balance coupled with an increased incidence of fatty liver during the periparturient period. The purpose of this study was to investigate the effect of hepatic steatosis on the expression of the sirtuin 1 (SIRT1)...
Olga V Nikolskaia et al.
The Journal of clinical investigation, 116(10), 2739-2747 (2006-09-26)
In this study we investigated why bloodstream forms of Trypanosoma brucei gambiense cross human brain microvascular endothelial cells (BMECs), a human blood-brain barrier (BBB) model system, at much greater efficiency than do T. b. brucei. After noting that T. b....
Asja Guzman et al.
Biomaterials, 115, 19-29 (2016-11-24)
Invasive breast cancer and other tumors of epithelial origin must breach a layer of basement membrane (BM) that surrounds the primary tumor before invading into the adjacent extracellular matrix. To analyze invasive strategies of breast cancer cells during BM breaching...
While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.
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