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P1860

Sigma-Aldrich

Protease Inhibitor Cocktail

DMSO solution, for the inhibition of serine, cysteine, aspartic proteases and aminopeptidases, for use in tissue culture media, DMSO solution

Synonym(s):

Protease Inhibitor Solution, protease inhibitor

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About This Item

EC Number:
MDL number:
UNSPSC Code:
12352200
NACRES:
NA.77

product name

Protease Inhibitor Cocktail, for use in tissue culture media, DMSO solution

Quality Level

form

DMSO solution

shipped in

dry ice

storage temp.

−20°C

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General description

Protease Inhibitor Cocktail demonstrated non-toxicity towards the following cell lines after 48 hours exposure:
A431, CHO, COS, HepG2, and HeLa adherent cell lines
Jurkat and HL-60 cell lines grown in suspension
The Protease Inhibitor Cocktail is a mixture of protease inhibitors designed to prevent proteolytic degradation of secreted proteins in tissue culture media.

Specificity

Inhibits serine, cysteine, aspartic proteases, and aminopeptidases

Application

Protease Inhibitor Cocktail has been used:
  • as a supplement in supernatants and lysates for ex vivo infection experiments using human precision-cut lung slices (PCLS)
  • to inhibit proteolysis by trypsin to prove that proteolysis is responsible for restoration of catalysis of corona-inhibited nanozymes
  • in trypsin solution for trypsin-inhibited studies
  • as a component in radioimmunoprecipitation assay (RIPA) buffer to lyse leukocytes for western blot

This product is specifically optimized for use in tissue culture media and can be added after 48 hours of exposure to fresh medium to ensure the continued inhibition of proteases.

Biochem/physiol Actions

Protease inhibitor cocktail is designed for use in tissue culture media. It is recommended as an additive to tissue culture media to safeguard against the degradation of secreted proteins originating from the cultured tissue.

Features and Benefits

  • Broad specificity
  • Non-toxic: After 48 hours exposure, the product is non-toxic to adherent cell lines A431, CHO, COS, HepG2 and HeLa; and to Jurkat and HL-60 cell lines grown in suspension.
  • Contains no metal chelators: Ensures compatibility with downstream applications
  • Convenient packaging: 1 mL in glass bottle
  • Easy to use: Use at a dilution of 1:200 or more in tissue culture media to prevent proteolytic degradation of secreted proteins.

Components

Aprotinin
Bestatin
E-64
Leupeptin
Pepstatin A

Caution

After 48 hours exposure, the product is non-toxic to adherent cell lines A431, CHO, COS, HepG2 and HeLa; and to Jurkat and HL-60 cell lines grown in suspension.

Quantity

Use at a dilution of 1:200 or more in tissue culture media to prevent proteolytic degradation of secreted proteins.

Physical form

Solution in DMSO (D 2650, Hybri-Max).

Other Notes

For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

188.6 °F

flash_point_c

87 °C


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.

Related Content

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

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