This protease inhibitor cocktail is designed for use in tissue culture media. Testing has not been conducted on mouse acinar cell primary culture. This product was found to be non-toxic to the following cell lines after 48 hours of exposure: A431, CHO, COS, HepG2, and HeLa adherent cell lines, as well as Jurkat and HL-60 cell lines grown in suspension. The suitability for specific applications should be determined empirically by the end user.
P1860
Protease Inhibitor Cocktail
DMSO solution, for the inhibition of serine, cysteine, aspartic proteases and aminopeptidases, for use in tissue culture media, DMSO solution
Synonym(s):
Protease Inhibitor Solution, protease inhibitor
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About This Item
Form:
DMSO solution
Solubility:
water: soluble
Storage temp.:
−20°C
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Product Name
Protease Inhibitor Cocktail, for use in tissue culture media, DMSO solution
Quality Level
form
DMSO solution
solubility
water: soluble
shipped in
dry ice
storage temp.
−20°C
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This Item | P8215 | P9599 | P8340 |
|---|---|---|---|
| form DMSO solution | form DMSO solution | form DMSO solution | form DMSO solution |
| Quality Level 200 | Quality Level 300 | Quality Level 300 | Quality Level 300 |
| shipped in dry ice | shipped in - | shipped in - | shipped in - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
General description
Protease Inhibitor Cocktail demonstrated non-toxicity towards the following cell lines after 48 hours exposure:
A431, CHO, COS, HepG2, and HeLa adherent cell lines
Jurkat and HL-60 cell lines grown in suspension
A431, CHO, COS, HepG2, and HeLa adherent cell lines
Jurkat and HL-60 cell lines grown in suspension
The Protease Inhibitor Cocktail is a mixture of protease inhibitors designed to prevent proteolytic degradation of secreted proteins in tissue culture media.
Application
Protease Inhibitor Cocktail has been used:
- as a supplement in supernatants and lysates for ex vivo infection experiments using human precision-cut lung slices (PCLS)[1]
- to inhibit proteolysis by trypsin to prove that proteolysis is responsible for restoration of catalysis of corona-inhibited nanozymes[2]
- in trypsin solution for trypsin-inhibited studies[2]
- as a component in radioimmunoprecipitation assay (RIPA) buffer to lyse leukocytes for western blot[3]
This product is specifically optimized for use in tissue culture media and can be added after 48 hours of exposure to fresh medium to ensure the continued inhibition of proteases.
Biochem/physiol Actions
Inhibits serine, cysteine, aspartic proteases, and aminopeptidases
Protease inhibitor cocktail is designed for use in tissue culture media. It is recommended as an additive to tissue culture media to safeguard against the degradation of secreted proteins originating from the cultured tissue.
Features and Benefits
- Broad specificity
- Non-toxic: After 48 hours exposure, the product is non-toxic to adherent cell lines A431, CHO, COS, HepG2 and HeLa; and to Jurkat and HL-60 cell lines grown in suspension.
- Contains no metal chelators: Ensures compatibility with downstream applications
- Convenient packaging: 1 mL in glass bottle
- Easy to use: Use at a dilution of 1:200 or more in tissue culture media to prevent proteolytic degradation of secreted proteins.
Physical form
Solution in DMSO (D 2650, Hybri-Max).
Preparation Note
Use at a dilution of 1:200 or more in tissue culture media to prevent proteolytic degradation of secreted proteins.
Other Notes
Aprotinin
Bestatin
E-64
Leupeptin
Pepstatin A
Bestatin
E-64
Leupeptin
Pepstatin A
For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
Disclaimer
After 48 hours exposure, the product is non-toxic to adherent cell lines A431, CHO, COS, HepG2 and HeLa; and to Jurkat and HL-60 cell lines grown in suspension.
related product
Product No.
Description
Pricing
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
188.6 °F
flash_point_c
87 °C
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Related Content
Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.
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I want to use this protease inhibitor cocktail in the context of mouse acinar cell primary culture for 7 days. What concentration of the Cocktail would you suggest?
1 answer-
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What are the concentrations of the individual protease inhibitor components in the cocktail?
1 answer-
The concentrations of the individual protease inhibitor components are considered proprietary and are not published.
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