Works great in immuno-precipitations from yeast extracts,
P8215
Protease Inhibitor Cocktail
DMSO solution, for the inhibition of serine, cysteine, aspartic and metalloproteases, for use with fungal and yeast extracts, DMSO solution
Protease Inhibitor Cocktail
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Synonym(s):
Protease Inhibitor Mix, Protease inhibitor
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 mL | Available to ship TODAYfromMILWAUKEE | $121.00 | |
| 5 mL | Available to ship TODAYfromMILWAUKEE | $403.00 |
About This Item
Form:
DMSO solution
Solubility:
water: soluble
Storage temp.:
−20°C
Product Name
Protease Inhibitor Cocktail, for use with fungal and yeast extracts, DMSO solution
Quality Level
form
DMSO solution
solubility
water: soluble
storage temp.
−20°C
General description
The Protease Inhibitor Cocktail for fungal and yeast extracts is a mixture of protease inhibitors in DMSO solution. The cocktail has been optimized and tested for use on fungal and yeast samples, specifically on Saccharomyces cerevisiae cells. One mL of the cocktail is recommended for the inhibition of proteases extracted from 20 g of yeast.
Application
Protease Inhibitor Cocktail has been used as a component in:
- FA buffer to wash spheroplasts to obtain an average DNA fragment size for chromatin immunoprecipitation (chip)
- potassium phosphate buffer (pbs) to digest chitin
- extraction buffer for grinding powdered mycelia for co-immunoprecipitation analysis
Biochem/physiol Actions
Inhibits serine, cysteine, aspartic, and metalloproteases.
Features and Benefits
- Broad specificity: inhibits a wide range of proteases, providing comprehensive protection to fungal and yeast extracts.
- Tested on Saccharomyces cerevisiae cells: the cocktail has been optimized for use on this commonly studied yeast strain.
- Convenient packaging: available in a 1 or 5 mL glass bottle for easy handling and storage.
- Ready-to-use solution: the cocktail is supplied in DMSO solution for immediate use in protease inhibition assays.
- Effective inhibition: each component in the cocktail has been carefully selected for its specific inhibitory properties, ensuring reliable and consistent results.
Physical form
Solution in DMSO
Preparation Note
One mL is recommended for the inhibition of proteases extracted from 20 g of yeast.
Other Notes
AEBSF, 100 mM
E-64, 1.4 mM
Pepstatin A, 2.2 mM
1,10-Phenanthroline, 500 mM
E-64, 1.4 mM
Pepstatin A, 2.2 mM
1,10-Phenanthroline, 500 mM
Mixture of protease inhibitors with broad specificity for the inhibition of serine, cysteine, aspartic and metallo-proteases. Contains 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF), pepstatin A, E-64, and 1,10-phenanthroline.
This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
1 of 1
This Item | |||
|---|---|---|---|
| form DMSO solution | form DMSO solution | form DMSO solution | form DMSO solution |
| Quality Level 300 | Quality Level 300 | Quality Level 200 | Quality Level 300 |
| solubility water: soluble | solubility water: soluble | solubility water: soluble | solubility water: soluble |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
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Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2
Storage Class
10 - Combustible liquids
flash_point_f
188.6 °F
flash_point_c
87 °C
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Related Content
Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.
Datasheet
A genome-wide screen reveals a role for the HIR histone chaperone complex in preventing mislocalization of budding yeast CENP-A
Ciftci-Yilmaz S, et al.
Genetics, 210(1), 203-218 (2018)
Elena Pérez-Nadales et al.
The Plant cell, 23(3), 1171-1185 (2011-03-29)
Fungal pathogenicity in plants requires a conserved mitogen-activated protein kinase (MAPK) cascade homologous to the yeast filamentous growth pathway. How this signaling cascade is activated during infection remains poorly understood. In the soil-borne vascular wilt fungus Fusarium oxysporum, the orthologous
Takashi Kubota et al.
Molecular & cellular proteomics : MCP, 10(7), M110-M110 (2011-04-21)
Yeast cells lacking Ctf18, the major subunit of an alternative Replication Factor C complex, have multiple problems with genome stability. To understand the in vivo function of the Ctf18 complex, we analyzed chromatin composition in a ctf18Δ mutant using the
Global Trade Item Number
| SKU | GTIN |
|---|---|
| P8215-5ML | 04061838446534 |
| P8215-2ML | 04061837258565 |
| P8215-1ML | 04061838446527 |