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P8340

Sigma-Aldrich

Protease Inhibitor Cocktail

for use with mammalian cell and tissue extracts, DMSO solution

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MDL number:
MFCD00677817
NACRES:
NA.77

biological source

bovine lung

Quality Level

300

form

DMSO solution

storage temp.

−20°C

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form

DMSO solution

form

DMSO solution

form

DMSO solution

form

ready-to-use solution

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

300

Quality Level

300

Quality Level

200

Quality Level

-

General description

Protease Inhibitor Cocktail for mammalian cell and tissue extracts, in DMSO solution. It contains six individual components for inhibiting serine, cysteine, and acid proteases, and aminopeptidases.

Specificity

Inhibits serine, cysteine, and acid proteases, and aminopeptidases.

Application

Ideal for use as a buffer component during sonication, lysis buffer, and RIPA buffer

Provides protection against endogenous enzyme activity in cell and tissue extracts

Biochem/physiol Actions

This mixture contains individual components, including AEBSF at 104 mM, Aprotinin at 80 μM, Bestatin at 4 mM, E-64 at 1.4 mM, Leupeptin at 2 mM and Pepstatin A at 1.5 mM. Each component has specific inhibitory properties. AEBSF and Aprotinin act to inhibit serine proteases, including trypsin, chymotrypsin, and plasmin amongst others. Bestatin inhibits aminpeptidases. E-64 acts against cystein proteases. Leupeptin acts against both serine and cystein proteases. Pepstatin A inhibits acid proteases.

Features and Benefits

Broad-spectrum inhibition: inhibits multiple classes of proteases and aminopeptidases

Stable storage: supplied as a solution in DMSO, allowing for long-term storage at -20°C

Consistent performance: ready-to-use solution

Components

AEBSF
Aprotinin
Bestatin
E-64
Leupeptin
Pepstatin A

Caution

This cocktail should be stored at -20 °C.

Preparation Note

One mL of this cocktail solution is recommended for the inhibition of endogenous enzymes found in 100 mL of lysate from 20 g of bovine liver or 10 mL of lysate from CHO cells. This product is supplied as a solution in DMSO.

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Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315 - H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup

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Megan D Schertzer et al.
Molecular cell, 75(3), 523-537 (2019-07-02)
Long noncoding RNAs (lncRNAs) cause Polycomb repressive complexes (PRCs) to spread over broad regions of the mammalian genome. We report that in mouse trophoblast stem cells, the Airn and Kcnq1ot1 lncRNAs induce PRC-dependent chromatin modifications over multi-megabase domains. Throughout the
Interaction of Huntington disease protein with transcriptional activator Sp1.
Li SH et al
Molecular and Cellular Biology, 22(5), 1277-1287 (2002)
Acceleration and persistence of neurofibrillary pathology in a mouse model of tauopathy following anesthesia.
Planel E et al
Faseb Journal, 23(8), 2595-2604 (2009)
Signaling through extracellular signal-regulated kinase is required for spermatogonial proliferative response to stem cell factor.
Dolci S et al
The Journal of Biological Chemistry, 276(43), 40225-40233 (2001)
A transgenic rat that develops Alzheimer's disease-like amyloid pathology, deficits in synaptic plasticity and cognitive impairment.
Liu L et al
Neurobiology of Disease, 31(1), 46-57 (2008)
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