DCTP-RO

dCTP

99% (dNTP), <0.9% (dNDP), solution, 100 mM

• Sinônimo(s): 2′-Deoxycytidine 5′-triphosphate disodium salt, dCTP-Na₂
• Fórmula empírica (Notação de Hill): C₉H₁₄N₃Na₂O₁₃P₃
• Número CAS: 102783-51-7
• Peso molecular: 511.12
• UNSPSC Code: 41106305
• PubChem Substance ID: 329798916
• MDL number: MFCD00084683
• NACRES: NA.54
• Assay: 99% (dNTP)
• Form: solution
• Storage temp.: −20°C

Propriedades

• description: PCR Grade, sodium salt
• assay: 99% (dNTP)
• form: solution
• usage: sufficient for 125,000 standard reactions (03732690001), sufficient for 31,250 standard reactions (11969021001), sufficient for 6,250 standard reactions (11934520001)
• packaging: pkg of 1,250 μL (11969021001 [100 mM]), pkg of 4 × 1,250 μL (03732690001 [4x100 mM]), pkg of 250 μL (11934520001 [100 mM])
• manufacturer/tradename: Roche
• concentration: <0.9% (dNDP), 100 mM
• technique(s): whole genome amplification: suitable
• storage temp.: −20°C
• SMILES string: [Na+].[Na+].NC1=NC(=O)N(C=C1)[C@H]2C[C@H](O)[C@@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)O2
• InChI: 1S/C9H16N3O13P3.2Na/c10-7-1-2-12(9(14)11-7)8-3-5(13)6(23-8)4-22-27(18,19)25-28(20,21)24-26(15,16)17;;/h1-2,5-6,8,13H,3-4H2,(H,18,19)(H,20,21)(H2,10,11,14)(H2,15,16,17);;/q;2*+1/p-2/t5-,6+,8+;;/m0../s1
• InChI key: ABWVCNMFYVEBIB-CDNBRZBRSA-L

Descrição

General description

dCTP, PCR Grade, is a 100 mM clear colorless solution of the sodium salt (pH 8.3). PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity. PCR Grade nucleotides contain at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP).

Application

2′-Deoxycytidine 5′-triphosphate disodium salt is one of the nucleoside triphosphates used for DNA polymerase driven reactions such as polymerase chain reaction, DNA sequencing and other molecular biology methods.

Physical form

solution of dCTP in water (pH 8.3).

Preparation Note

Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP, dTTP (10 mM, each); (e.g., for the preparation of 100 μl nucleotide mix add 10 μl of dATP, dCTP, dGTP, dTTP (each) to 60 μl Water, PCR Grade).

Analysis Note

Function tested in RT-PCR. Tested for absence of contaminating deoxyribonucleases, ribonucleases and nicking activities according to the current Quality Control procedures.

Other Notes

For general laboratory use.

Informações de segurança

• Classe de armazenamento: 12 - Non Combustible Liquids
• O que está acontecendo?: WGK 1
• Ponto de inflamação (°F): does not flash
• Ponto de inflamação (°C): does not flash