TRYPSEQ-RO

Trypsin Sequencing Grade

from bovine pancreas

• Sinônimo(s): trypsin
• UNSPSC Code: 12352204
• Número CE: 3.4.21.4 (BRENDA, IUBMB)
• NACRES: NA.54
• Specific activity: ≥80 units/mg protein
• Biological source: bovine pancreas

Propriedades

• biological source: bovine pancreas
• form: lyophilized (salt-free)
• specific activity: ≥80 units/mg protein
• mol wt: M_r 23.5 kDa
• packaging: pkg of 4 × 100 μg (11047841001), pkg of 4 × 25 μg (11418475001)
• manufacturer/tradename: Roche
• optimum pH: 8.0
• storage temp.: 2-8°C

Descrição

General description

Trypsin Sequencing Grade is isolated from bovine pancreas as a highly purified and specific protease. Trypsin Sequencing Grade is a serine endopeptidase.[1] It specifically cleaves peptide bonds at the carboxylic side of the basic amino acids Arg and Lys. Amide and ester bonds of Arg and Lys are also cleaved.[2]

Application

Trypsin Sequencing Grade is used to to digest proteins in solution, in gels or on blotting membranes.
The enzyme is used for protein-structure elucidation, tryptic mapping, fingerprinting, sequence analysis, and translocation studies.[3] Trypsin Sequencing Grade generates glycopeptides from purified glycoproteins and is suited for the digestion of proteins in polyacrylamide gels.

Biochem/physiol Actions

The specificity of Trypsin Sequencing Grade is verified with the oxidized B-chain of insulin (insulin B_ox) as substrate. High concentrations of Trypsin Sequencing Grade (1 part by weight enzyme with 18 parts by weight insulin B_ox) are incubated for 18 hours to detect traces of chymotrypsin impurities.

Specificity (HPLC, with Insulin B_ox):
Cleavage after 1 hour: ≥90%;
Unspecific cleavage products after 18 hours: ≤10%

Preparation Note

Stabilizers: Trypsin is stable in 4 M.
Working concentration: 1/100 to 1/20 of the protein by weight (in solution); 1-5 μg/100 μl (for in-gel digest)
Storage conditions (working solution): A solution in 0.01% trifluoroacetic acid (TFA), (v/v) or 1 mM HCl may be used for one week at maximum, if stored at 2 to 8 °C. By incubation of proteins in solution at neutral to slightly basic pH-values partial autolysis might occur. For this application, Roche recommends Trypsin, Modified, Sequencing Grade.

Reconstitution in acid is necessary for stability of solution: 0.01% TFA (v/v), 1 mM HCl or 0.1% acetic acid are recommended.

Analysis Note

Purity: Free of impurities that may interfere with the separation of peptides in reversed-phase HPLC.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Informações de segurança

• pictograms: GHS07,GHS08
• signalword: Danger
• hcodes: H315,H319,H334,H335
• pcodes: P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Classe de armazenamento: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: does not flash
• flash_point_c: does not flash