Merck
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F2012

Sigma-Aldrich

Anti-Mouse IgG (whole molecule)–FITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Todas as fotos(1)
Sinônimo(s):
Goat Anti-Mouse IgG (whole molecule)–Fluorescein isothiocyanate
Número MDL:
MFCD00162642

fonte biológica

goat

Nível de qualidade

200

conjugado

FITC conjugate

forma do anticorpo

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

forma

buffered aqueous solution

technique(s)

direct immunofluorescence: 1:64 (minimum)

temperatura de armazenamento

2-8°C

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Anti-Mouse IgG (whole molecule)–FITC antibody produced in goat affinity isolated antibody, buffered aqueous solution

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F2012

Anti-Mouse IgG (whole molecule)–FITC antibody produced in goat

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F6258

Anti-Rat IgG (whole molecule)–FITC antibody produced in goat

Anti-Mouse IgG (whole molecule)–FITC antibody produced in goat affinity isolated antibody, buffered aqueous solution

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F0257

Anti-Mouse IgG (whole molecule)–FITC antibody produced in goat

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Sigma-Aldrich

F9006

Anti-Mouse IgG (whole molecule)–FITC antibody produced in goat

conjugate

FITC conjugate

conjugate

FITC conjugate

conjugate

FITC conjugate

conjugate

FITC conjugate

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

IgG fraction of antiserum

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

technique(s)

direct immunofluorescence: 1:64 (minimum)

technique(s)

direct immunofluorescence: 1:32

technique(s)

direct immunofluorescence: 1:32

technique(s)

direct immunofluorescence: 1:64

Descrição geral

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by disulfide bond. It mainly helps in immune defense. It is a glycoprotein and a major class of immunoglobulin. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.
The product binds all mouse Igs.

Aplicação

Anti-Mouse IgG (whole molecule)−FITC antibody has been used:
  • in immunofluorescence
  • in immunohistochemistry
  • in immunofluorescence staining
  • in immunofluorescence microscopy
  • in confocal imaging

Anti-Mouse IgG (whole molecule)-FITC antibody produced in goat was used for FACS analysis of DF3 antigen on breast cancer cell lines.

Ações bioquímicas/fisiológicas

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of FITC to Anti-Mouse IgG (whole molecule) antibody allows for the visualization of protein by fluorescent microscopy.

Outras notas

Antibody adsorbed with human serum proteins.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Nota de preparo

Adsorbed to reduce background staining with human samples

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de classe de armazenamento

10 - Combustible liquids

WGK

nwg

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

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Exemplo

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Número do produto
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25G
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Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

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The
The Laboratory Rat (2005)
Adhesion and differentiation of human mesenchymal stem cells on plasma-functionalized graphenes with different feeding gases
Baik K Y, et al.
Carbon, 77(10), 302-310 (2014)
L Chen et al.
The Journal of clinical investigation, 96(6), 2775-2782 (1995-12-01)
The high molecular weight mucin-like glycoprotein, DF3 (MUC1), is overexpressed in the majority of human breast cancers. Here we demonstrate that replication defective recombinant adenoviral vectors, containing the DF3 promoter (bp -725 to +31), can be used to express beta-galactosidase
Differential cellular effects of electroporation and electrochemotherapy in monolayers of human microvascular endothelial cells
Meulenberg C J W, et al.
Testing, 7(12), e52713-e52713 (2012)
Stimulation of intracellular sphingosine-1-phosphate production by G-protein-coupled sphingosine-1-phosphate receptors
zu H D M, et al.
European Journal of Pharmacology, 414(2-3), 145-154 (2001)
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