P4591
Pyruvate Oxidase from microorganisms
lyophilized powder, ≥1.5 U/mg
Sinônimo(s):
Pyruvate:oxygen oxidoreductase (phosphorylating)
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| Tamanho/SKU | Disponibilidade | Preço |
|---|---|---|
100 units | Verificar disponibilidade no carrinho | R$ 2.689,00 |
Sobre este item
Número CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-644-5
MDL number:
Número CE:
Specific activity:
≥1.5 U/mg
R$ 2.689,00
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Assistência Técnica
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Estamos aqui para ajudarGeneral description
Isoelectric point : 4.3
Michaelis constant : 3.4 X 10-4M (Pyruvate)
Inhibitors : Fe++,Zn++,Cu++,Ag+,Hg++
Optimum pH : 5.7
Optimum temperature : 65oC
pH Stability : pH 5.7 - 6.5 (25oC, 20hr)
Thermal stability : below 45oC (pH 6.0, 15min)
Michaelis constant : 3.4 X 10-4M (Pyruvate)
Inhibitors : Fe++,Zn++,Cu++,Ag+,Hg++
Optimum pH : 5.7
Optimum temperature : 65oC
pH Stability : pH 5.7 - 6.5 (25oC, 20hr)
Thermal stability : below 45oC (pH 6.0, 15min)
Application
Pyruvate Oxidase from microorganisms has been used in the Amplex Red-based fluorescence assay for pyruvate. It has also been used in developing near real-time continuous detection system for pyruvate.
Pyruvate Oxidase (PoxB) converts pyruvate directly to acetate and CO2. It is used to study pyruvate metabolism. It is used to study aerobic metabolism of bacterium, such as Lactobacillus plantarumand Streptococcus pneumoniae. Pyruvate Oxidase is used for enzymatic determination of pyruvate, GOT, and GPT in clinical analysis.
Biochem/physiol Actions
Pyruvate Oxidase oxidises pyruvate to form acetate, hydrogen peroxide and carbondioxide. Pyruvate oxidase requires flavin adenine dinucleotide (FAD), thiamine pyrophosphate (TPP) and magnesium as cofactors for its catalytic activity. Pyruvate oxidase is activated by the cofactor thiamine.
Pyruvate Oxidase consists of four subunits with identical molecular weights. PoxB reacts with certain aldehydes and phosphate can be replaced by arsenate. Oxygen as well as several artificial compounds can function as electron acceptors. Pyruvate Oxidase is activated by phospholipids as well as monomeric and micellar amphiphiles.
Physical form
Lyophilized powder containing FAD and sugar as stabilizer
Other Notes
One unit will produce 1.0 μmole of H2O2 per min during the conversion of pyruvate and phosphate to acetylphosphate and CO2 at pH 5.7 at 37 °C.
1 of 1
Este Item | |||
|---|---|---|---|
| description lyophilized powder, ≥1.5 U/mg | description lyophilized powder, ≥50 units/mg protein, recombinant, expressed in E. coli | description lyophilized powder, ≥35 units/mg protein (biuret) | description aqueous solution, ≥30 units/mg protein (biuret) |
| specific activity ≥1.5 U/mg | specific activity ≥50 units/mg protein | specific activity ≥35 units/mg protein (biuret) | specific activity ≥30 units/mg protein (biuret) |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form aqueous solution |
| mol wt ~260 kDa | mol wt 64 kDa | mol wt - | mol wt 62 kDa |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −70°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Palavra de aviso
Danger
Códigos de risco
Declarações de precaução
Hazard Classifications
Resp. Sens. 1
Classe de armazenamento
11 - Combustible Solids
O que está acontecendo?
WGK 3
Ponto de inflamação (°F)
Not applicable
Ponto de inflamação (°C)
Not applicable
EPI (equipamento de proteção individual)
Eyeshields, Gloves, type N95 (US)
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