I bought this kit as replacement of NA0410-1Kt which I was waiting for 1 year and still wasn't able to get due to delay. Plex15 is significantly more expensive, uncomfortable to work with and yields less plasmid DNA. I regret I had to waste my time and money on it.
PLEX15
GenElute™ Endotoxin-free Plasmid Maxiprep Kit
sufficient for 15 purifications
Recursos de sustentabilidade
Sinônimo(s):
GenElute™ Endotoxin-free Plasmid Kits, Gen Elute
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| Tamanho/SKU | Disponibilidade | Preço |
|---|---|---|
1 kit | Verificar disponibilidade no carrinho | R$ 3.373,00 |
Sobre este item
NACRES:
NA.52
UNSPSC Code:
41105501
R$ 3.373,00
Verificar disponibilidade no carrinho
Assistência Técnica
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sufficient for 15 purifications
Quality Segment
sustainability
Sustainability features
technique(s)
DNA purification: suitable
test parameters
: <2 hr kit run time for up to 12 preps, sample volume: 25-200 mL bacterial culture
storage temp.
15-25°C
General description
The GenElute Endotoxin-free Plasmid Kit offers a simple, rapid, cost effective method for purifying plasmid DNA with ≤0.1 EU/μg DNA for high efficiency transfection. Endotoxins (also known as lipopolysaccharides or LPS) are often co-purified with plasmid DNA and significantly reduce transfection efficiencies in endotoxin-sensitive cell lines.
Recombinant E. coli is harvested from an overnight culture by centrifugation and subjected to a modified alkaline-SDS lysis procedure to produce a cleared lysate. Endotoxins are removed from the cleared lysate with simple extraction-phase separation steps. Plasmid DNA is further purified by adsorption onto silica in the presence of high salts. After a simple spin-wash step, the bound plasmid DNA is eluted in endotoxin-free water. Up to 250 ug of endotoxin-free plasmid DNA may be prepared from 5-40 mL of culture.
The recovered plasmid DNA is predominately in its supercoiled form. The DNA is ready for immediate use in downstream applications such as transfection, restriction digestion, cloning, sequencing, and PCR amplification.
Recombinant E. coli is harvested from an overnight culture by centrifugation and subjected to a modified alkaline-SDS lysis procedure to produce a cleared lysate. Endotoxins are removed from the cleared lysate with simple extraction-phase separation steps. Plasmid DNA is further purified by adsorption onto silica in the presence of high salts. After a simple spin-wash step, the bound plasmid DNA is eluted in endotoxin-free water. Up to 250 ug of endotoxin-free plasmid DNA may be prepared from 5-40 mL of culture.
The recovered plasmid DNA is predominately in its supercoiled form. The DNA is ready for immediate use in downstream applications such as transfection, restriction digestion, cloning, sequencing, and PCR amplification.
This product aligns with one or more of the 12 Principles of Green ChemistryThis product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
Application
The kit has been used to purify plasmid DNA for high efficiency transfection.
Features and Benefits
- High transfection efficiency and yield
- Purify up to 250 μg endotoxin-free plasmid DNA (≤0.1 EU/μg DNA)
- Fast and simple allowing up to 12 preps in less than 2 hours
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
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Este Item | |||
|---|---|---|---|
| description sufficient for 15 purifications | description sufficient for 25 purifications | description sufficient for 25 preparations | description sufficient for 10 purifications |
| technique(s) DNA purification: suitable | technique(s) DNA purification: suitable | technique(s) DNA extraction: suitable | technique(s) DNA purification: suitable |
| usage sufficient for 15 purifications | usage sufficient for 25 purifications | usage sufficient for 25 preparations | usage sufficient for 10 purifications |
| test parameters sample volume: 25-200 mL bacterial culture, : <2 hr kit run time for up to 12 preps | test parameters sample volume: 150 mL bacterial culture | test parameters sample volume: 150 mL bacterial culture | test parameters sample volume: 50 mL bacterial culture |
| storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C |
| sustainability Sustainability features | sustainability Sustainability features | sustainability Sustainability features | sustainability Sustainability features |
| greener alternative category | greener alternative category | greener alternative category | greener alternative category |
Palavra de aviso
Danger
Códigos de risco
Declarações de precaução
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Órgãos-alvo
Central nervous system
Ponto de inflamação (°F)
Not applicable
Ponto de inflamação (°C)
Not applicable
WGK
WGK 3
Classe de armazenamento
10 - Combustible liquids
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