Todas as fotos(1)



GlycoProfile I, In-Gel Deglycosylation Kit

Número EC:

Nível de qualidade


prazo de validade

≥1 yr at 2‑8 °C

temperatura de armazenamento



The GlycoProfile Enzymatic In-gel N-Deglycosylation Kit is optimized to provide a convenient, reproducible method to N-deglycosylate and digest protein samples from one dimensional (1D) and two dimensional (2D) polyacrylamide gel pieces for subsequent mass spectrometry or HPLC analysis.
This procedure is suitable for Coomassie® Brilliant Blue and colloidal Coomassie stained gels. Silver stained gels may also be used if properly destained.


The kit includes Proteomics Grade PNGase F and trypsin enzymes necessary for N-linked deglycosylation and tryptic digestion, respectively. The samples can then be desalted and concentrated for analysis by MALDI-TOF MS or electrospray MS with subsequent database searching.


Each kit contains reagents sufficient to deglycosylate and digest up to 10 samples.

Informações legais

GlycoProfile is a trademark of Sigma-Aldrich Co. LLC

Somente componentes do kit

Nº do produto

  • Acetonitrile, ≥99.93%, BioSyn® 50 mL

Componentes do kit também disponíveis separadamente

Nº do produto

  • P7367PNGase F from Elizabethkingia meningoseptica, BioReagent, ≥95% (SDS-PAGE), for proteomics 50 U

  • T6567Trypsin from porcine pancreas, Proteomics Grade, BioReagent, Dimethylated 20 μg

  • Trypsin Solubilization Reagent

  • I0408Invertase Glycoprotein Standard, BioReagent, from Saccharomyces cerevisiae, for proteomics

Palavra indicadora


Classificações de perigo

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3


Respiratory system

Código de classe de armazenamento

3 - Flammable liquids



Ponto de fulgor (ºF)

35.6 °F

Ponto de fulgor (ºC)

2.0 °C

Certificado de análise

Insira o número de lote para pesquisar o Certificado de análise (COA).

Certificado de origem

Insira o número de lote para pesquisar o Certificado de origem (COO).


O-Linked Glycan Strategies

There is no enzyme comparable to PNGase F for removing intact O-linked sugars. Monosaccharides must be sequentially hydrolyzed by a series of exoglycosidases until only the Gal-b(1-3)-GalNAc core remains. O-Glycosidase can then remove the core structure intact with no modification of the serine or threonine residue.

Glycoprotein Deglycosylation

Information about Glycoprotein deglycosylation. The diversity of oligosaccharide structures, both O-linked and N-linked, often results in heterogeneity in the mass and charge of glycoproteins.

Deglycosylation Kits

Deglycosylation Kits

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica