The microprotein kit TP0200 is not intended for use above 1 mg/ml
TP0200
Total Protein Kit, Micro-Lowry, Onishi & Barr Modification
sufficient for ~50 manual assays
Sinônimo(s):
Total Protein Assay Kit
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Sobre este item
NACRES:
NA.32
UNSPSC Code:
12352202
eCl@ss:
32150312
Preço e disponibilidade não estão disponíveis no momento.
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wavelength 500-750nm
Quality Segment
usage
sufficient for ~50 manual assays
protein detection level measuring range
0-1 mg/mL
parameter
18-26 °C temperature, 200 μL sample volume
storage temp.
2-8°C
General description
The biuret and Lowry procedures are used for protein determination. The former is widely used for clinical assays. The latter, though more sensitive, is used for investigative work and is limited by poor stability of combined reagents, non-reproducibility of color, especially at low protein concentration, and nonlinear chromogenic response with protein concentration. Ohnishi and Barr modified the biuret reagent for the Lowry procedure, thereby simplifying it while improving the stability of the combined reagent.
Application
Total Protein Kit, Micro-Lowry, Onishi & Barr Modification has been used to determine total soluble protein concentrations:
- in the dialyzates/extracts of gliadins
- in the extracts of flour (Avena sativa and Triticum durum) and seeds (Chenopodium quinoa, Salvia hispanica L.)
- in the stored samples of rat liver microsomal fractions
Biochem/physiol Actions
Colorimetric, Endpoint.assay. Ohnishi and Barr′s modification of micro Lowry method. According to procedure, dilute biuret reagent reacts with peptide bonds to yield a purple-blue complex, the color of which is intensified by the addition of phenol reagent. Absorbance, read at 550-750 nm, is used to determine results from a standard curve.
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Este Item | |||
|---|---|---|---|
| description sufficient for ~50 manual assays | description - | description - | description suitable for determination of total protein by Lowry method, 1.9-2.1 N |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| usage sufficient for ~50 manual assays | usage sufficient for ~200 assays | usage - | usage - |
| parameter 18-26 °C temperature, 200 μL sample volume | parameter - | parameter - | parameter - |
| storage temp. 2-8°C | storage temp. - | storage temp. - | storage temp. room temp |
| protein detection level measuring range 0-1 mg/mL | protein detection level measuring range - | protein detection level measuring range - | protein detection level measuring range - |
| description wavelength 500-750nm | description - | description - | description - |
Palavra de aviso
Danger
Códigos de risco
Declarações de precaução
Hazard Classifications
Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1
Classe de armazenamento
8A - Combustible corrosive hazardous materials
Ponto de inflamação (°F)
Not applicable
Ponto de inflamação (°C)
Not applicable
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does it works for samples with 11 mg/ml?
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