The control beads are utilized to demonstrate consistency across the assay plate. Each bead is conjugated with varying amounts of immunoglobulin, resulting in different levels of relative MFI. The negative control bead is not conjugated with immunoglobulin and serves as a blank. These controls are not intended for analysis but should indicate a trend, with the negative bead reading at background levels, control bead 1 exhibiting high MFI, and control beads 2 and 3 showing lower readings.
HC19SERA1-85K
MILLIPLEX® SARS-CoV-2 Antigen Panel 1 IgA
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Über diesen Artikel
NACRES:
NA.47
UNSPSC Code:
12352200
To order a Milliplex® kit, please search for your analyte of interest.
product line
Milliplex®
species reactivity
human
technique(s)
multiplexing: suitable
detection method
fluorometric (Luminex® xMAP®)
storage temp.
2-8°C
General description
"The SARS-CoV-2 coronavirus is the pathogenic cause of the human disease COVID-19. The virus particle consists of RNA encapsulated by the nucleocapsid (N) protein which is enveloped by a membrane (M) protein. The corona-like spike proteins surrounding this inner core consist of subunits S1, S2 and the receptor binding domain which binds to the human cellular ACE-2 receptor. Upon binding, the virus enters the cell, takes over the cellular machinery, reproduces, and the viral contagion continues. Viral structural proteins offer antigenic potential for an immune response. Upon viral infection the humoral immune system responds first by producing the immunoglobulin IgM, followed later by IgG which can convey long lasting immunity. IgA, a mucosal immune response, is also found in blood.By testing for IgM, IgG, and IgA in human serum and plasma, researchers may identify those who have been exposed to the SARS-CoV-2 virus and mounted some level of immune response. Researchers may further understand the immune response over the course of infection and recovery from COVID-19."MILLIPLEX® SARS-CoV-2 Antigen Panel 1 IgA is a 4-plex kit to be used for the simultaneous analysis of human serum or plasma samples for IgA antibodies which recognize any or all of the following analytes: SARS-CoV-2 Spike S1, SARS-CoV-2 Spike S2, SARS-Cov-2 RBD, Sars-CoV-2 N.
Application
The SARS-CoV-2 Antigen Panel 1 IgA is a bead-based multiplex assay based on the Luminex® xMAP technology which enables the simultaneous analysis of human samples for IgA antibodies which recognize any of the following analytes: SARS-CoV-2 Spike S1, SARS-CoV-2 Spike S2, SARS-Cov-2 RBD, Sars-CoV-2 N. This is assay yields results in Median Fluorescence Intensity (MFI).Four assay control beads are included in the base kit.This is a one day assay.This assay requires 25 μL of 1:100 diluted plasma or serum.Analytes available:SARS-CoV-2 Nucleocapsid Protein (N);SARS-CoV-2 Receptor Binding Domain (RBD);SARS-CoV-2 Spike Protein Subunit 1 (S1);SARS-CoV-2 Spike Protein Subunit 2 (S2)
Features and Benefits
Design your multiplex kit by choosing available analytes within this panel.
Packaging
96-well plate
Preparation Note
Recommended storage for kit components is 2 - 8°C.
Legal Information
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Luminex is a registered trademark of Luminex Corporation
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Dieser Artikel | |||
|---|---|---|---|
| description - | description - | description - | description - |
| species reactivity human | species reactivity human | species reactivity human | species reactivity - |
| technique(s) multiplexing: suitable | technique(s) multiplexing: suitable | technique(s) multiplexing: suitable | technique(s) single molecule counting: suitable |
| detection method fluorometric (Luminex® xMAP®) | detection method fluorometric (Luminex® xMAP®) | detection method fluorometric (Luminex® xMAP®) | detection method - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| product line Milliplex® | product line Milliplex® | product line Milliplex® | product line SMC® |
Signalwort
Warning
Gefahrencodes
Sicherheitshinweise
Hazard Classifications
Eye Irrit. 2
Lagerklasse
10 - Combustible liquids
WGK
WGK 2
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I am using the HC19SERA1-85 K SARs Ag Panel IgA kit. WHy are the controls also magnetic beads? I do not understand how this will work if I add the controls to my Plasma sample? Won't this give me false positives?
1 answer-
Helpful?
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We are running the SARS-CoV-2 IgG, IgA, and IgM kits for the first time on the MagPix. What analysis settings should be used since the kit does not include a standard? Are there any units that should be entered?
1 answer-
For Qualitative Assays, the Analysis Type is "None" in xPonent. There are no units to be entered. Median values are used for the data comparison between treatment groups.
Helpful?
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