CS0780
β-N-Acetylglucosaminidase Assay Kit
sufficient for 50 reactions (1 mL), sufficient for 500 reactions (100 μL)
Synonym(e):
Beta-NAG Activity Assay Kit
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UNSPSC Code:
12161503
EC Number:
222-398-7
NACRES:
NA.84
Technischer Dienst
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Unterstützung erhaltenQuality Segment
usage
sufficient for 50 reactions (1 mL), sufficient for 500 reactions (100 μL)
shipped in
wet ice
storage temp.
2-8°C
Gene Information
human ... NAGLU(4669)
Application
The kit provides the reagents required for a fast and convenient detection of β-N-Acetylglucosaminidase activity in cell lysates, tissue homogenates or purified enzyme. It is a useful tool for the detection of lysosomes in fractionated cell/tissue samples.
Biochem/physiol Actions
N-acetyl-D-glucosaminidase (NAG) in mammals is a lysosomal enzyme, which takes part in the intracellular degradation of glycolipids and glycoproteins. High activities of this enzyme have been detected in human kidney, lung and liver lysosomes. Elevated levels of serum NAG are associated with certain forms of cancer.
Analysis Note
The kit assay is based on the hydrolysis of NAG substrate (NP-GLcNAc) by the enzyme. The enzymatic hydrolysis of the substrate liberates p-nitrophenylate ion. The reaction product is detected colorimetrically at 405 nm.
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Signalwort
Warning
Gefahrencodes
Sicherheitshinweise
Hazard Classifications
Carc. 2 - Eye Irrit. 2 - STOT RE 2 Oral
Zielorgane
Liver,Kidney
WGK
WGK 3
Lagerklasse
10 - Combustible liquids
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