CS2000

SARS-CoV-2 (COVID-19) Inhibitors Screening Kit 

Based on SPIKE - ACE2 Binding Assay, Colorimetric, sufficient for 200 tests

• Synonym(e): ACE2, COVID-19, S protein, SARS-CoV-2, Spike protein
• NACRES: NA.84
• UNSPSC Code: 12352200

Eigenschaften

• Quality Segment: 200
• shipped in: ambient
• storage temp.: 2-8°C

Beschreibung

General description

designed to simplify the identification and characterization of SARS-CoV-2 inhibitors.

Application

The SARS-CoV-2 Inhibitors Screening Kit is a convenient tool, designed to simplify the identification and characterization of SARS-CoV-2 inhibitors.The crutial interaction between the SARS-CoV-2 and human cells accrues through the protein-protein interaction of human receptor ACE2 and viral protein Spike – S protein. The receptor binding domain (RBD) of S protein is the domain that specifically binds to ACE2. Our SARS-CoV-2 Inhibitors Screening Kit includes both human ACE protein and viral RBD of S protein. The kit is based on a colorimetric ELISA, where biotinylated ACE2 (ACE2-biotin) and Extravidin^®-peroxidase are used to detect the ACE2:RBD interaction. One molecule of Extravidin^® binds four molecules of biotin by a non-covalent interaction that is essentially irreversible. Binding visualization is achieved by using o-Phenylenediamine dihydrochloride (OPD), a peroxidase substrate with absorbance at 450 nm.

Inhibitors and neutralizing antibodies against RBD that interfere with the ACE2:RBD interaction will result in reduced colorimetric signal.

Biochem/physiol Actions

SARS-CoV-2 - Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) belongs to Coronaviridae (CoVs) - a large family of viruses that usually cause mild to moderate upper-respiratory tract diseases.1 CoVs are single-stranded RNA viruses. They have a high mutation rate and are therefore highly diverse.1 Their hosts include human and non-human mammals, as well as birds.1

SARS-CoV-2 infects cells which express angiotensin converting enzyme 2 (ACE2).2 ACE2 binds to the viral envelope-associated spike (S) protein as a cellular receptor, following proteolytic cleavage of both S and ACE2 by serine proteases.3 S protein is believed to be the target of neutralizing antibodies because it is the main trans-membrane glycoprotein responsible for receptor-binding and virion entry.4

The S protein is a trimeric protein. During the binding to ACE2, S protein undergoes a substantial structural rearrangement to facilitate the fusion of the viral membrane with the host cell membrane.5 The receptor binding domain (RBD) of S protein is the domain that specifically binds to ACE2. RBD is the major target for neutralizing antibodies for coronaviruses6, and anti-RBD antibodies likely correlate with virus neutralization.7

Features and Benefits

Our SARS-CoV-2 Inhibitors Screening Kit will support your research, enabling to screen and discover inhibitors as potential drugs for COVID-19

Supportive calculator (Click here to download a calculator excel file)

Legal Information

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• pictograms: GHS08,GHS05,GHS07,GHS09
• signalword: Danger
• hcodes: H302,H317,H318,H335,H341,H351,H411
• pcodes: P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Skin Sens. 1 - STOT SE 3
• target_organs: Respiratory system
• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 3