This product is a liquid medium. The density is not reported. The 1L volume weighs approximately 1 kilogram. The total weight of the packaged 1L size is 1.1 kg. The total weight of the packaged 500 mL size is 578 g.
D6429
DMEM - high glucose
With sodium bicarbonate, ʟ-glutamine and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture
Dulbecco’s Modified Eagle’s Medium - high glucose
1 of 5 reviewers received a sample product or took part in a promotion
Synonym(e):
DME, DMEM
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UNSPSC-Code:
12352207
NACRES:
NA.71
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Unterstützung erhaltenProduktname
Dulbecco Modifiziertes Eagle-Medium – hoher Glucosegehalt, With 4500 mg/L glucose, L-glutamine, sodium pyruvate, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Qualitätsniveau
Sterilität
sterile-filtered
Form
liquid
Methode(n)
cell culture | mammalian: suitable
Verunreinigungen
endotoxin, tested
Komponenten
HEPES: no
NaHCO3: yes
phenol red: yes
sodium pyruvate: yes
L-glutamine: yes
glucose: high
Versandbedingung
ambient
Lagertemp.
2-8°C
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1 of 4
Dieser Artikel | D6546 | D5796 | D1145 |
|---|---|---|---|
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| form liquid | form liquid | form liquid | form liquid |
| components HEPES: no | components NaHCO3: yes | components NaHCO3: yes | components L-glutamine: no |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
Allgemeine Beschreibung
Dieses DMEM-High-Glucose-Medium ist ein einfach konzentriertes Vollmedium mit Natriumpyruvatzusatz. Es unterscheidet sich außerdem von der ursprünglichen DMEM-High-Formulierung darin, dass Pyridoxal durch Pyridoxin ersetzt wird. Pyridoxal ist ein instabiler Medienbestandteil.
Anwendung
Dulbecco′s Modified Eagle′s Medium (DMEM) ist eine Modifikation des Basal Medium Eagle (BME) mit vierfacher Konzentration der Aminosäuren und Vitamine. Die ursprüngliche Formulierung enthielt 1000 mg/l Glucose und diente zur Kultivierung von embryonalen Mäusezellen. Seitdem wurde es auf verschiedene Arten modifiziert, um damit Primärkulturen von Mäuse- und Hühnerzellen sowie verschiedene normale und transformierte Zellen kultivieren zu können. Jedes dieser Medien enthält eine andere Kombination von L-Glutamin und Natriumpyruvat. Außerdem wurde die Glucosekonzentration auf 4500 mg/l erhöht, dieses Medium wird als „DMEM/High“ bezeichnet.
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Produkt-Nr.
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Preisangaben
Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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How much does D6429-1L weigh? Thank you!
1 answer-
Helpful?
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Hi, What is the concentration of endotoxin detected in DMEM high glucose media D6429?
1 answer-
This product has an endotoxin specification of ≤ 1 EU/ml or 0.1 ng/mL. The lot specific values are reported in the Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/d6429#product-documentationHelpful?
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What is the concentration of Sodium Bicarbonate and Sodium Pyruvate in it?
1 answer-
This formulation contains 3.7 g/L sodium bicarbonate and 110 mg/L sodium pyruvate. The composition of this product is published on page 3 of the product information sheet. Please see the link below to access this document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/318/256/d6429for.pdf
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After opening a bottle of medium: how long can I use this bottle ?(stored at 4C°)
1 answer-
Provided that the product is handled and stored properly and has not been contaminated, the medium will be stable up to the expiration date. This date is provided on the label and the lot-specific Certificate of Analysis.
Helpful?
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I would like to inquire about the amino acid formulation in DMEM (#6429, #6546). Specifically, could you provide information on the concentration of aspartate and glutamate in these formulations? Thank you very much.
1 answer-
The composition of this product is published on page 3 of the product information sheet. Please see the link below to access this document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/318/256/d6429for.pdf
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