The specific formulation of this product is considered proprietary. The components are Recombinant Human Insulin, Recombinant Human Transferrin, Sodium Selenite, D-Glucose, Sodium Phosphate Monobasic, Potassium Chloride, Sodium Chloride, and Sodium Bicarbonate.
SCM05
AOF ITS Supplement (100X), 100ml | SCM055
AOF ITS supplement is an animal-origin-free product that contains human recombinant insulin, transferrin along with sodium selenite and is used for the serum-free culture of numerous cell types.
Sinonimo/i:
ITS, Insulin Transferrin Selenite
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10 ML
CHF 161.00
100 ML
CHF 960.00
Informazioni su questo articolo
Codice UNSPSC:
12352205
eCl@ss:
32160801
NACRES:
NA.75
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Stato
liquid
Concentrazione
100 ×
tecniche
cell culture | mammalian: suitable
cell culture | stem cell: suitable
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Questo articolo | I2521 | I3146 | I2771 |
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable, cell culture | stem cell: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable, single cell analysis: suitable | technique(s) cell culture | mammalian: suitable |
| concentration 100 × | concentration - | concentration - | concentration - |
| form liquid | form liquid | form liquid | form liquid |
| Quality Level 100 | Quality Level 500 | Quality Level 500 | Quality Level 300 |
Descrizione generale
EMD Millipore’s AOF ITS (100X) supplement is an animal-origin-free product that contains human recombinant insulin, human recombinant transferrin along with sodium selenite.
Undefined animal derived media supplements, such as fetal bovine serum, have recently been an area of concern for many cell culturists due to their increased costs, lot to lot variability and increased regulation worldwide. The elimination of fetal bovine serum from cell culture media provides a more defined enviroment to study basic biological questions. Serum-free and reduced serum formulations often require purified forms of the factors normally supplied by serum that are suitable for in vitro growth and differentiation studies such as insulin, transferrin and selenite.
ITS supplements have been proven to replace serum and grow the following cells in serum free or reduced serum conditions: CHO, HEK293, fibroblasts, hybridoma, VERO cells, Hela, HT29, MDCK, Mesenchymal, IPS Cells, Cardiac, kidney, keratinocyte, embryonic, neural stem cell, hepatocytes.
Undefined animal derived media supplements, such as fetal bovine serum, have recently been an area of concern for many cell culturists due to their increased costs, lot to lot variability and increased regulation worldwide. The elimination of fetal bovine serum from cell culture media provides a more defined enviroment to study basic biological questions. Serum-free and reduced serum formulations often require purified forms of the factors normally supplied by serum that are suitable for in vitro growth and differentiation studies such as insulin, transferrin and selenite.
ITS supplements have been proven to replace serum and grow the following cells in serum free or reduced serum conditions: CHO, HEK293, fibroblasts, hybridoma, VERO cells, Hela, HT29, MDCK, Mesenchymal, IPS Cells, Cardiac, kidney, keratinocyte, embryonic, neural stem cell, hepatocytes.
Applicazioni
EMD Millipore’s AOF ITS (100X) supplement is an completely animal-origin-free product
Research Category
Stem Cell Research
Stem Cell Research
Stato fisico
Product is made in an Earle′s Balanced Salt Solution and filtered through a 0.22 micron membrane before vialing. Solution is a light pink clear solution that is free of particulates.
Nota sulla preparazione
Store at 2-8°C. Further dilutions should be made into appropriate buffer and/or medium to which protein has been added, such as DMEM/1% Human Serum Albumin or equivalent.
Risultati analitici
Control
VERO cells
VERO cells
Tested for the ability to support the proliferation of VERO cells in serum free conditions over a 3 day time period.
pH: 7.6-8.0.
Endotoxin levels are ≤ 1 EU/mg.
pH: 7.6-8.0.
Endotoxin levels are ≤ 1 EU/mg.
Altre note
All components are derived using AOF (Animal-Origin-Free) reagents and protocols.
Recombinant Human Insulin is derived from Pichia pastoris (yeast).
Recombinant Human Transferrin is derived from Oryza sativa (rice).
Sodium Selenite is a synthetic chemical.
Recombinant Human Insulin is derived from Pichia pastoris (yeast).
Recombinant Human Transferrin is derived from Oryza sativa (rice).
Sodium Selenite is a synthetic chemical.
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Acute Tox. 4 Oral
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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