The Amicon Ultra 0.5 products are similar in size to a 1.5 mL Eppendorf tube. Refer to the User Guide for the dimensions. It is recommended to spin in a centrifuge below the maximum speed of 14,000 x g. Conversion to x g should be done either by calculation or by using an electronic switch on the centrifuge, rather than using r.p.m. for speed.
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Materiali
polypropylene cap
polypropylene tube
regenerated cellulose membrane
styrene-butadiene housing
Livello qualitativo
Sterilità
non-sterile
Nome Commerciale
Amicon®
Confezionamento
pkg of 24 pkg
pkg of 500 pkg
pkg of 8 pkg
pkg of 96 pkg
Produttore/marchio commerciale
Amicon® Ultra
sustainability
Greener Alternative Product
Parametri
0.5 mL sample volume
tecniche
protein extraction: suitable
protein purification: suitable
Lunghezza
4.99 cm (1.96 in.)
Diametro
10.8 mm
Superficie filtrante
1 cm2
Volume
0.5 mL
Volume d’esercizio
≤500 μL
0.5 mL
Dimensione pori
50 kDa MWCO
Categoria alternativa più verde
Condizioni di spedizione
ambient
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Questo articolo | UFC205024 | UFC8050 | UFC9050 |
|---|---|---|---|
| parameter 0.5 mL sample volume | parameter 2 mL sample volume | parameter 4 mL sample volume | parameter 15 mL sample volume |
| material polypropylene cap, regenerated cellulose membrane, polypropylene tube, styrene-butadiene housing | material polypropylene tube (for collection), regenerated cellulose membrane, styrene-butadiene housing | material polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing | material polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing |
| manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra |
| pore size 50 kDa MWCO | pore size 50 kDa MWCO | pore size 50 kDa MWCO | pore size 50 kDa MWCO |
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| product line Amicon® | product line Amicon® | product line Amicon® | product line Amicon® |
Descrizione generale
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 30-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.
® ®
Applicazioni
- ®
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
- Purification of macromolecular components found in tissue culture extracts or cell lysates, primer removal, linkers, or macromolecular labels from a reaction mix, and protein removal prior to HPLC
- Desalting, buffer exchange, and protein dialysis
Caratteristiche e vantaggi
- ®
- μμ
- Concentration factor 25x to 30x
- High sample recovery (greater than 90% of dilute starting solution)
- Fast processing time: da 10 a 30C.
- Reverse spin capability provides consistent recoveries
Stato fisico
Altre note
Note legali
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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Articoli
Metodi di filtrazione e selezione di filtri a membrana per la purificazione e produzione di nanoparticelle
Explore ultrafiltration membrane principles and benefits for protein sample preparation, including solvent exchange and sample concentration.
Commercially available protein labeling kits provide researchers with the flexibility to customize their own immunoassay detection panels.
Protein purification is simpler with Amicon Pro. The device delivers consistent sample preparation for more reliable recovery, uncompromised purity and easier data generation.
Protocolli
Detergents are frequently used to solubilize proteins and nucleic acids during purification, but the presence of detergents may interfere with downstream analyses.
If an antibody must be purified from a small sample volume (0.5-10 mL), then the Amicon® Pro Purification System is recommended.
Contenuto correlato
Proteinkonzentration ohne Verlust. Gewinnen Sie Proteine zuverlässig zurück, wenn Sie mit Amicon® Ultra Filtern konzentrieren.
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Is it compatible with the microcentrifuge machine (max 20000 r.p.m.) for 1.5 mL eppendorf tubes?
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UFC505096について、耐用gを教えてください。
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This product is not assigned an expiration date but end users should check stock after 3 years. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.
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