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Merck

UFC5050

Filtri centrifughi Amicon® Ultra, MWCO 50 kDa

greener alternative

sample volume 0.5 mL, regenerated cellulose membrane, MWCO 50 kDa

Sinonimo/i:

Concentratore centrifugo, MWCO di 50 kDa, volume del campione 0,5 mL


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Informazioni su questo articolo

Codice UNSPSC:
41104916
eCl@ss:
36100101
NACRES:
NB.24

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Materiali

polypropylene cap
polypropylene tube
regenerated cellulose membrane
styrene-butadiene housing

Livello qualitativo

Sterilità

non-sterile

Nome Commerciale

Amicon®

Confezionamento

pkg of 24 pkg
pkg of 500 pkg
pkg of 8 pkg
pkg of 96 pkg

Produttore/marchio commerciale

Amicon® Ultra

sustainability

Greener Alternative Product

Parametri

0.5 mL sample volume

tecniche

protein extraction: suitable
protein purification: suitable

Lunghezza

4.99 cm (1.96 in.)

Diametro

10.8 mm

Superficie filtrante

1 cm2

Volume

0.5 mL

Volume d’esercizio

≤500 μL
0.5 mL

Dimensione pori

50 kDa MWCO

Categoria alternativa più verde

Condizioni di spedizione

ambient

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Questo articolo
UFC205024UFC8050UFC9050
parameter

0.5 mL sample volume

parameter

2 mL sample volume

parameter

4 mL sample volume

parameter

15 mL sample volume

material

polypropylene cap, regenerated cellulose membrane, polypropylene tube, styrene-butadiene housing

material

polypropylene tube (for collection), regenerated cellulose membrane, styrene-butadiene housing

material

polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing

material

polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing

manufacturer/tradename

Amicon® Ultra

manufacturer/tradename

Amicon® Ultra

manufacturer/tradename

Amicon® Ultra

manufacturer/tradename

Amicon® Ultra

pore size

50 kDa MWCO

pore size

50 kDa MWCO

pore size

50 kDa MWCO

pore size

50 kDa MWCO

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

product line

Amicon®

product line

Amicon®

product line

Amicon®

product line

Amicon®

Descrizione generale

® >

The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 30-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.

® ®
We are committed to bringing you Greener Alternative Products, which have been re-engineered to enable more sustainable research solutions. The inner filtration device is made from bio-circular material allocated using the ISCC mass balance approach. Click here for more information.

Applicazioni

  • ®
  • Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
  • Purification of macromolecular components found in tissue culture extracts or cell lysates, primer removal, linkers, or macromolecular labels from a reaction mix, and protein removal prior to HPLC
  • Desalting, buffer exchange, and protein dialysis

Caratteristiche e vantaggi

  • ®
  • μμ
  • Concentration factor 25x to 30x
  • High sample recovery (greater than 90% of dilute starting solution)
  • Fast processing time: da 10 a 30C.
  • Reverse spin capability provides consistent recoveries

Stato fisico

Codice colore: Trasparente

Altre note

Sostituisce: 42415

Note legali

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Articoli

Metodi di filtrazione e selezione di filtri a membrana per la purificazione e produzione di nanoparticelle

Explore ultrafiltration membrane principles and benefits for protein sample preparation, including solvent exchange and sample concentration.

Commercially available protein labeling kits provide researchers with the flexibility to customize their own immunoassay detection panels.

Protein purification is simpler with Amicon Pro. The device delivers consistent sample preparation for more reliable recovery, uncompromised purity and easier data generation.

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Protocolli

Detergents are frequently used to solubilize proteins and nucleic acids during purification, but the presence of detergents may interfere with downstream analyses.

If an antibody must be purified from a small sample volume (0.5-10 mL), then the Amicon® Pro Purification System is recommended.

Contenuto correlato

Proteinkonzentration ohne Verlust. Gewinnen Sie Proteine zuverlässig zurück, wenn Sie mit Amicon® Ultra Filtern konzentrieren.

Questions

1–2 of 2 Questions  
  1. Is it compatible with the microcentrifuge machine (max 20000 r.p.m.) for 1.5 mL eppendorf tubes?

    1 answer
    1. The Amicon Ultra 0.5 products are similar in size to a 1.5 mL Eppendorf tube. Refer to the User Guide for the dimensions. It is recommended to spin in a centrifuge below the maximum speed of 14,000 x g. Conversion to x g should be done either by calculation or by using an electronic switch on the centrifuge, rather than using r.p.m. for speed.

      Helpful?

  2. UFC505096について、耐用gを教えてください。

    1 answer
    1. This product is not assigned an expiration date but end users should check stock after 3 years. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.

      Helpful?

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