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Merck

P1754

TWEEN® 80

viscous liquid

TWEEN® 80

Sinonimo/i:

TWEEN® 80, Monooleato di polietilenglicole sorbitano, Monooleato di poliossietilene sorbitano, POE (20) sorbitano monooleato, Polisorbato 80

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25 ML

CHF 41.40

500 ML

CHF 110.00

1 L

CHF 172.00

1 GAL

CHF 473.00

CHF 41.40

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Informazioni su questo articolo

Numero CAS:
9005-65-6
Numero MDL:
MFCD00082107
Codice UNSPSC:
12161900
NACRES:
NA.21

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Servizio Tecnico
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Origine biologica

Porcine (tallow)
bovine (tallow)

Livello qualitativo

200

Descrizione

non-ionic

Stato

viscous liquid

PM

micellar avg mol wt 79,000
average mol wt 1310

Composizione

Oleic acid, ≥58.0% (balance primarily linoleic, palmitic, and stearic acids)

Numero d'aggregazione

60

CMC

0.012 mM (20-25°C)

Viscosità

400-620 mPa.s

Temp. transizione

cloud point 65 °C

Solubilità

water: soluble, clear to slightly hazy, colorless to yellow

Densità

1.064 g/cm3

HLB

15

applicazioni

cell analysis

Stringa SMILE

CCCCCCCC/C=C/CCCCCCCC(=O)OCCOCC(C1C(CC(O1)OCCO)OCCO)OCCO

InChI

1S/C32H60O10/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-30(36)40-25-24-37-27-29(39-22-19-34)32-28(38-21-18-33)26-31(42-32)41-23-20-35/h9-10,28-29,31-35H,2-8,11-27H2,1H3/b10-9+
RGPBUVUVZKQNHD-MDZDMXLPSA-N

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1 of 4

Questo articolo
P8074P4780STS0204
TWEEN® 80 viscous liquid

Sigma-Aldrich

P1754

TWEEN® 80

TWEEN® 80 BioXtra, viscous liquid

Sigma-Aldrich

P8074

TWEEN® 80

TWEEN® 80 suitable for cell culture, suitable for insect cell culture, viscous liquid

Sigma-Aldrich

P4780

TWEEN® 80

TWEEN® 80 viscous liquid

Sigma-Aldrich

STS0204

TWEEN® 80

form

viscous liquid

form

viscous liquid

form

viscous liquid

form

viscous liquid

aggregation number

60

aggregation number

60

aggregation number

-

aggregation number

-

application(s)

cell analysis

application(s)

-

application(s)

-

application(s)

-

biological source

Porcine (tallow), bovine (tallow)

biological source

-

biological source

-

biological source

-

viscosity

400-620 mPa.s

viscosity

-

viscosity

-

viscosity

300—500 cP(25 °C)

density

1.064 g/cm3

density

1.06 g/mL at 25 °C

density

1.06 g/mL at 25 °C (lit.), 1.07 g/cm3 at 25 °C (lit.)

density

1.064 g/cm3

Descrizione generale

Il Tween® 80 è un detergente e tensioattivo non ionico, che può essere classificato come un estere del sorbitano e dell′acido oleico. È un composto versatile, usato comunemente in svariate applicazioni biologiche, come la lisi cellulare, l′isolamento dei nuclei e il frazionamento cellulare. In più, il Tween 80 trova impiego nella stabilizzazione delle proteine e d è d′ausilio negli studi sulle proteine di membrana. La sua efficacia discende dal suo appartenere alla classe dei polisorbati, composti definiti strutturalmente dalla combinazione di esteri oleici del sorbitolo e del sorbitano con catene di polietilenglicoli. Queste specificità strutturali contribuiscono alle proprietà emulsionanti del Tween 80 e della sua molteplicità di applicazioni.

Applicazioni

TWEEN® 80 è stato usato come soluzione di eccipiente per specifiche iniezioni in topi e ratti.[1][2] È stato usato per la determinazione dell′attività dell′esterasi nelle cellule batteriche.[3]
È un detergente non ionico usato per l′estrazione selettiva delle proteine e per l′isolamento dei nuclei da linee cellulari di mammifero.

Azioni biochim/fisiol

TWEEN® 80 è un tensioattivo polisorbato utile nella stabilizzazione delle proteine e negli studi sulle membrane proteiche. È usato regolarmente per l′estrazione delle proteine di membrana e per l′isolamento dei nuclei dalle cellule.[4][5][6]

Caratteristiche e vantaggi

  • Agente bagnante e disperdente eccellente
  • Tensioattivo spiccatamente versatile per le vostre ricerche di biologia cellulare e biochimica.

Altre note

Per ulteriori informazioni sulla nostra gamma di Composti biochimici, compilare questo modulo.

Note legali

TWEEN is a registered trademark of Croda International PLC

Prodotto comparabile

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Determinazione del prezzo

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

>235.4 °F - closed cup

Punto d’infiammabilità (°C)

> 113 °C - closed cup

Dispositivi di protezione individuale

Eyeshields, Gloves

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Certificati d'analisi (COA)

Lot/Batch Number
BCCN6549
BCCN4705
BCCM9515
BCCM7843
BCCM6321

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Questions

1–10 of 11 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. This material is free of residual solvents?

    1 answer

    1. This product is not tested for residual solvents. Should this be a requirement, see product 817061, which is tested for Ethylene Oxide, 1,4-Dioxane, and Ethylene glycol. Please see the link below to review this item:
      https://www.sigmaaldrich.com/product/mm/817061

      Helpful?

  4. What is the boiling point of this specific Tween 80 product?

    1 answer

    1. This product is not tested for boiling point. However, various reference sources cite a boiling point of approximately 100°C.

      Helpful?

  5. Hello, Is there an expiration date or retest date for this product?

    1 answer

    1. This product is not assigned an expiration date. It is assigned a recommended retest date of approximately 4 years after the quality release date. A recommended retest date is the time during which the product is expected to remain within established stability specifications, provided that it has been stored under defined conditions. After the Retest Date, product samples should be examined to ensure that the product is still in compliance with the established specifications.

      For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/US/en/life-science/quality-and-regulatory-management/quality-services.

      Helpful?

  6. Is Product P1754, TWEEN® 80, cell culture tested?

    1 answer

    1. No, P1754 is not cell culture tested, but product P4780 is cell culture tested TWEEN 80.

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  7. What is the Critical Micelle Concentration (CMC) of Product P1754, TWEEN® 80?

    1 answer

    1. The CMC value is 13-15 mg/Liter.

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  8. Is Product P1754, TWEEN® 80, sterile?

    1 answer

    1. The product is not sterile. Autoclaving a solution of TWEEN 80 is generally not advised. Sterile filtration is more easily done if the liquid is warmed to about 40°C and alternate portions of hot distilled water and TWEEN 80 are poured through the 0.22 μm filter. The TWEEN 80 will blend and remain in solution.

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  9. Is it normal for the color of Product P1754, TWEEN® 80, to change from lot to lot?

    1 answer

    1. Yes. According to Sigma-Aldrich's specifications, Product No. P1754 can appear bright yellow to yellow with a green cast.  Please see our Specification Sheet for more information.

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  10. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

1–10 of 11 Questions  
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  1. 1 Ratings-only review

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

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