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Merck

R1755

Rhodamine B isothiocyanate

BioReagent, mixed isomers, suitable for protein labeling

Sinonimo/i:

RBITC

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100 MG

CHF 177.00

500 MG

CHF 488.00

1 G

CHF 851.00

CHF 177.00


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Informazioni su questo articolo

Formula empirica (notazione di Hill):
C29H30ClN3O3S
Numero CAS:
Peso molecolare:
536.08
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12352116
EC Number:
253-248-9
MDL number:

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Nome del prodotto

Rhodamine B isothiocyanate, BioReagent, mixed isomers, suitable for protein labeling

InChI key

YVSWPCCVTYEEHG-UHFFFAOYSA-N

InChI

1S/C29H29N3O3S.ClH/c1-5-31(6-2)20-10-13-23-26(16-20)35-27-17-21(32(7-3)8-4)11-14-24(27)28(23)22-12-9-19(30-18-36)15-25(22)29(33)34;/h9-17H,5-8H2,1-4H3;1H

SMILES string

[Cl-].CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccc(cc4C(O)=O)N=C=S)=[N+](\CC)CC)c1

description

labeling efficiency with bovine albumin > 70%

product line

BioReagent

Quality Level

form

powder

mol wt

536.08 g/mol

composition

9-[2-carboxy-5(or 6)-isothiocyanatophenyl]-3,6-bis(diethylamino)xanthylium chloride, ≤100%

color

purple to red-brown

solubility

methanol: 10 mg/mL (dark red to very dark red and dark purple to very dark purple)

fluorescence

λex 543 nm; λem 580 nm in methanol

suitability

suitable for protein labeling

storage temp.

−20°C

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Questo articolo
28392483689R9379
Rhodamine B suitable for fluorescence

Sigma-Aldrich

83689

Rhodamine B

suitability

suitable for protein labeling

suitability

-

suitability

-

suitability

-

fluorescence

λex 543 nm; λem 580 nm in methanol

fluorescence

-

fluorescence

λex 553 nm; λem 627 nm in methanol

fluorescence

-

storage temp.

−20°C

storage temp.

room temp

storage temp.

-

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

product line

BioReagent

product line

-

product line

-

product line

-

description

labeling efficiency with bovine albumin > 70%

description

-

description

-

description

-

Application

Rhodamine B isothiocyanate is used for the following applications:
  • Rhodamine isothiocyanate labeling[1]
  • Rhodamine-labeled synthetic APF[2]
  • Preparations of protein and fluorescent conjugates[3]
  • Fluorescent red dye[4]

General description

Rhodamine B isothiocyanate (RhBITC) is an organic fluorescence dye and is widely used as a fluorescent tag in a variety of biological applications.[5] RhBITC is adsorbed onto Ag on silica or polystyrene beads. It exhibits not only a strong surface-enhanced Raman scattering (SERS) signal but also a measurable amount of fluorescence. The RhBITC-modified Ag-deposited silica or polystyrene beads disperse well in ethanol. They are also readily coated in water with polyelectrolytes for their further derivatization with biological molecules of interest that can bind to target molecules.[6]

pictograms

Health hazardExclamation mark

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Classe di stoccaggio

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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Jun-Sung Kim et al.
Journal of veterinary science, 7(4), 321-326 (2006-11-16)
Biocompatible silica-overcoated magnetic nanoparticles containing an organic fluorescence dye, rhodamine B isothiocyanate (RITC), within a silica shell [50 nm size, MNP@SiO2(RITC)s] were synthesized. For future application of the MNP@SiO2(RITC)s into diverse areas of research such as drug or gene delivery
Control Over Aragonite Crystal Nucleation and Growth: An In Vitro Study of Biomineralization.
Yael Levi et al.
Chemistry (Weinheim An Der Bergstrasse, Germany), 4 (1998)
Kwan Kim et al.
Biosensors & bioelectronics, 24(7), 1864-1869 (2008-11-05)
Rhodamine B isothiocyanate (RhBITC) is a prototype dye molecule that is widely used as a fluorescent tag in a variety of biological applications. We report in this work that once RhBITC is adsorbed onto Ag on silica or polystyrene beads
S K Williams et al.
Proceedings of the National Academy of Sciences of the United States of America, 78(4), 2393-2397 (1981-04-01)
Microvessels isolated from rat epididymal fat exhibit differential vesicular ingestion rates for unmodified and non-enzymatically glycosylated rat albumin. While unmodified rat albumin is excluded from ingestion by endothelial micropinocytic vesicles, glycosylated albumin is avidly taken up by endocytosis. Interaction of
Mengying Zhang et al.
Lab on a chip, 10(9), 1199-1203 (2010-04-15)
We report a simple methodology to fabricate PDMS multi-layer microfluidic chips. A PDMS slab was surface-treated by trichloro (1H,1H,2H,2H-perfluorooctyl) silane, and acts as a reusable transferring layer. Uniformity of the thickness of the patterned PDMS layer and the well-alignment could

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Questions

  1. Hello, which form does this product come in? powder or liquid please. I would also like to know if it can be conjugated with chitosan polymers (nanomicelles)

    1 answer
    1. The product is supplied as a powder.
      Rhodamine B Isothiocyanate can be used for various applications. The only suitability test performed during quality control testing is for labelling efficiency with Bovine Serum Albumin. There is no suitability testing or protocol available for conjugating chitosan polymers. A search of the literature will be required to locate a protocol.

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