Determination of flurbiprofen in human plasma by gas chromatography with mass spectrometry and its pharmacokinetics.

Determination of flurbiprofen in human plasma by gas chromatography with mass spectrometry and its pharmacokinetics.

Journal of AOAC International (2014-08-26)

Bilal Yilmaz, Huseyin Sahin, Vedat Akba, Ali Fuat Erdem

ABSTRACT

This paper describes a GCIMS method for the determination of flurbiprofen in human plasma. Flurbiprofen and internal standard ibuprofen were extracted from plasma by using a liquid-liquid extraction method. Derivatization was carried out using N-Methyl-N-(trimethylsilyl)trifluoroacetamide. The calibration curve was linear between the concentration range of 0.10 and 5.0 microg/mL. Intraday and interday precision values for flurbiprofen in plasma were less than 5.49%, and accuracy (relative error) was better than 5.33%. The extraction recoveries of flurbiprofen from human plasma were between 93.6 and 98.6%. The LOD and LOQ of flurbiprofen were 0.03 and 0.10 microg/mL, respectively. This assay was applied to determine the pharmacokinetic parameters of flurbiprofen in healthy Turkish volunteers who had been given 100 mg of flurbiprofen.

MATERIALI

Numero di prodotto

Marchio

Descrizione del prodotto

34851

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Acetonitrile, suitable for HPLC, gradient grade, ≥99.9%

34856

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Diclorometano, suitable for HPLC, ≥99.8%, contains amylene as stabilizer

319902

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Etilacetato, ACS reagent, ≥99.5%

34998

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Acetonitrile, HPLC Plus, ≥99.9%

34858

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Etilacetato, suitable for HPLC, ≥99.7%

D65100

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Diclorometano, contains 40-150 ppm amylene as stabilizer, ACS reagent, ≥99.5%

C2432

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Cloroformio, contains 100-200 ppm amylenes as stabilizer, ≥99.5%

650463

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650498

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34859

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Esano, suitable for HPLC, ≥97.0% (GC)

360457

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Acetonitrile, ACS reagent, ≥99.5%

650528

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319988

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Cloroformio, contains ethanol as stabilizer, ACS reagent, ≥99.8%

366927

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139386

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Esano, ReagentPlus^®, ≥99%

270997

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270504

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271004

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1-butanolo, 99.9%

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