CRISPR Cas9 Proteins & Gene Editing
THE ULTIMATE SUITE OF CRISPR CAS9 PROTEIN ESSENTIALS AND SERVICES
Advances in genome engineering are enabling researchers to achieve unprecedented breakthroughs, from curing genetic disorders to developing disease-resistant crops. Our reliable CRISPR Cas9 protein essentials and tools deliver complete support for every step of CRISPR-based gene editing research from bench-top to larger scales. Target a single gene with zinc finger nucleases. Choose from two different PURedit® Cas9 Cytosine Base Editor Kits for C to T substitutions, optimized to unique editing windows for challenging edits. Screen a whole genome with fully customizable CRISPR products.
From CRISPR Cas9 proteins to synthetic guide RNAs to lentiviral products for CRISPR based screening, we have the ultimate suite of products for every step in your CRISPR-based gene editing research.
The Sigma-Aldrich® Advanced Genomics portfolio delivers the ultimate suite of genome engineering tools and services to unlock your research potential. Whether you are looking to knockout, knock-in, knockdown, or overexpress your targets, our comprehensive suite of CRISPR gene-editing tools and services will take your research Beyond the Bench.
Products
Popular Filters
Cas9-GFP Protein
from Streptococcus pyogenes, fused with enhanced GFP, recombinant, expressed in E. coli, 3X NLS
Cas9 Protein
from Streptococcus pyogenes, recombinant, expressed in E. coli, 1X NLS
X-tremeGENE™ siRNA Transfection Reagent
Polymer reagent for delivering siRNA to common cell lines
dCas9-3XFLAG™-Biotin Protein
from Streptococcus pyogenes with D10A and H840A mutations, recombinant, expressed in E. coli, 1X NLS
CMV-CAS9-2A-GFP Plasmid
Cas9-D10A Nickase Protein
from Streptococcus pyogenes with D10A mutation, recombinant, expressed in E. coli, 1X NLS
eSpCas9-GFP Protein
from Streptococcus pyogenes with mutations conferring enhanced specificity, fused with enhanced GFP, recombinant, expressed in E. coli, 3X NLS
Cas9 plasmid
eSpCas9 Protein
from Streptococcus pyogenes with mutations conferring enhanced specificity, recombinant, expressed in E. coli, 1X NLS
Cas9 mRNA
CRISPR Plant
CRISPR Cas9-D10A Nickase mRNA
CAS9 Blasticidin Lenti Particles
Sigma CRISPR dCas9-p300 Activator Expression Plasmid
Cas9 GFP Lentiviral Particles
Cas9-GFP Lenti Plasmid
FnCas9 Protein
from Francisella novicida, recombinant, expressed in E. coli, 1X NLS
CompoZr® Custom Zinc Finger Nuclease (ZFN)
ZFN plasmid and mRNA
SygRNA™ Cas9 Synthetic Modified tracrRNA
CRISPR Cas9-D10A Nickase Plasmid
Learn how PURedit® Cas9 Cystosine Base Editor Kits help provide the right edit, right now.
Best in Class Cas9 Proteins including PURedit® Cas9 Cytosine Base Editor Protein Kits
Sigma-Aldrich® offers multiple Cas9 variants (wild-type, enhanced specificity, nickase, GFP-fused, catalytically inactive) in several formats (plasmid, lentivirus, lyophilized protein). Our newest offering, PURedit® Cas9 Cytosine Base Editor protein kits, allow for defined cytosine base editing with two enzymes engineered by our experts for convenient RNP delivery, designed for success.
Guaranteed CRISPR gRNA
Every SygRNA® synthetic single guide RNA (sgRNA), crRNA, and tracrRNA is synthesized using our proprietary process and reagents from the highest quality materials available. Editing activity is assured regardless of your target, cell type, or application, and is backed by the most comprehensive guarantee in the industry. Take advantage of our free custom CRISPR design tools application where you can design your own guides for all type of CRISPR applications, including cytosine base editing with our PURedit® Cas9 Precision and Flexible Cytosine Base Editors.
CRISPR Inhibition and Activation (CRISPRi and CRISPRa)
Gain even greater insights into complex biological networks using Sigma-Aldrich® CRISPR activation and CRISPR inhibition libraries for gene knockdown and overexpression experiments. Pooled CRISPRi and SAM CRISPRa lentiviral libraries are available off-the-shelf or customized to your specific needs.
CRISPR Screening with 10x Genomics Compatibility
Sigma-Aldrich® offers the only 10x Genomics Certified Compatible lentiviral pools for CRISPR single-cell screening. Our optimized, custom lentiviral vectors are fully compatible with Chromium Single Cell Gene Expression v3 and v3.1 (Next GEM) solution utilizing Feature Barcode technology.
We’re Here to Help
Whether you need help designing your CRISPR experiment, require assistance troubleshooting, or simply want to better understand how you can apply gene editing methods in your research, Sigma-Aldrich® Advanced Genomics is here to guide you.
Wondering if CRISPR is right for your project?
Related Resources
- Brochure: Molecular Essentials Guide
Learn how our comprehensive molecular tools and reagents provide precise, ready-to-use solutions for your workflows.
- Article: CRISPR Cas9 Proteins
The largest selection of quality Cas9 proteins provides ideal CRISPR reagents for your experiments, ensuring efficient and straightforward gene editing.
- Article: CRISPR Cas9 Protein Product Selection Guide
A convenient CRISPR Cas9 protein selection guide to assist with your CRISPR RNP, lentivirus, mRNA, plasmids, vectors and control needs.
- CRISPR Use License Agreement
This Product and its use are the subject of one or more of the following patents controlled by The Broad Institute, Inc. (BROAD), the Massachusetts Institute of Technology (MIT), or the President and Fellows of Harvard College (HARVARD): U.S. Patent Nos. 8,697,357; 8,771,945, and any substitutions, divisions, continuations, reissues, renewals, re-examinations or extensions, and corresponding foreign patent applications and patents.
- Article: Cas9-Mediated Recombineering Enhances Metabolic Production of Kdo2-Lipid A by E. coli
In this article, we present an application of our novel E. coli CRISPR/Cas-mediated Lambda-Red (λ-Red) homologous recombination (HR) vector system, which facilitates gene editing through the homology-directed repair (HDR) of double-stranded DNA breaks (DSBs) created by Cas9 endonuclease, using either ssDNA or dsDNA as an editing template.
- Article: Ribonucleoprotein (RNP) Protocols Using Synthetic sgRNAs and Cas9 proteins
Combine guaranteed sgRNAs with our comprehensive range of CRISPR products and tools, including Cas9 and delivery reagents, for efficient genome modification with higher specificity.
- Article: Genome Editing in Plants with CRISPR/Cas9
Zinc finger nucleases (ZFNs), were introduced by Sigma-Aldrich less than 8 years ago, but in that time the technology of targeted genome editing has advanced rapidly. Most recently, the discovery of the CRISPR/Cas9 pathway has accelerated interest in this field, opening up new possibilities for research and development.
- Article: PURedit™ CRISPR Synthetic Guide RNAs and Cas9 Protein
Guaranteed PURedit™ CRISPR synthetic gRNAs and Cas9 protein offer industry-leading on-site cutting and specificity.
- eBook: Gene Editing 101
Learn how to understand, design and perform CRISPR experiments, as well as design gRNA, choose a Cas9 format, screen with CRISPR, use advanced CRISPR formats, and much more…
- Article: Blue-White Screening & Protocols for Colony Selection
Our popular blue-white screening for colony selection technical resource hub, transfection reagent selection guide and protocols.
- Article: Introduction to Cell Transfection
An overview on cell transfection vs transduction methods, our cell transfection reagent selection guide and protocols.
- Protocol: Western Blotting
Western blotting protocol that includes technical information supporting the following: Sample preparation, membrane transfer/tank transfer, transfer stack assembly, buffers, immunodetection, and a troubleshooting guide.
- Article: CRISPRi & CRISPRa Genetic Screening
Explore optimized CRISPR libraries for gene modulation experiments, offering off-the-shelf or customizable options.
- Article: Single-Cell Resolution Discovery
CRISPR lentiviral screening libraries, partnered with 10x Genomics, offer powerful research tools for pooled screening.
- Flyer: Determining Which Screening Option is Right for Your Experiment
Designing a screening experiment can be daunting with all the technologies and formats available. With the largest portfolio of screening tools on the market, we can help ensure you have the right tools at the right scale to meet your goals.
- Flyer: CRISPRi Whole Genome and Non-Coding Libraries
Lentiviral libraries and pools to take your research beyond the bench.
- Flyer: Whole Genome SAM CRISPRa Libraries
A complete kit for genome scale activation.
- Flyer: CRISPR Dual Guide Lentiviral Vectors
Double the editing power with dual gRNAs and integrated 10x genomics compatibility for single-cell analysis.
Web Tools
- PCR Master Mix Calculator
Perform calculations for large scale PCR reactions and ensure success of scaled up reactions with this online PCR Master Mix Calculator.
- CRISPR Design Tool
Design your guide RNA for standard Cas9 editing and PURedit® Cas9 Cytosine Base Editor Proteins.
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