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D6434

DMEM/F-12

Name: DMEM/F-12
Brand: SIGMA

With HEPES, sodium bicarbonate and sodium pyruvate, without ʟ-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

Synonyme(s) :

DME/F-12, mélange 1/1

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A propos de cet article

UNSPSC Code:

12352207

NACRES:

NA.75

MDL number:

MFCD00217417

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Propriétés

Nom du produit

Milieu de Eagle modifié par Dulbecco (DMEM)/Mélange nutritif F-12 de Ham, With 15 mM HEPES and sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

Quality Segment

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

L-glutamine: no
phenol red: no
NaHCO₃: yes
HEPES: 15 mM
sodium pyruvate: 0.055 g/L
glucose: 3.15 g/L

shipped in

ambient

storage temp.

2-8°C

Description

General description

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Pyridoxal, which is an aldehyde, has been replaced with pyridoxine. This modification has been shown to improve the stability of the medium. Suitable for growth of a wide range of cell types in low serum conditions. Absence of phenol red makes this a preferred medium for clonal and stem cell culture.

Application

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used to culture:

bovine endometrial epithelial cells
cancer stem cells
breast cancer cell line

Preparation Note

Supplement with 0.365 g/L L-glutamine

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1 of 1

Cet article	D2906	D6421	FG4815
Sigma-Aldrich D6434 Milieu de Eagle modifié par Dulbecco (DMEM)/Mélange nutritif F-12 de Ham	Sigma-Aldrich D2906 Milieu de Eagle modifié par Dulbecco (DMEM)/Mélange nutritif F-12 de Ham	Sigma-Aldrich D6421 Milieu de Eagle modifié par Dulbecco (DMEM)/Mélange nutritif F-12 de Ham	Sigma-Aldrich FG4815 Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham
description With 15 mM HEPES and sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture	description With L-glutamine and 15 mM HEPES, without sodium bicarbonate and phenol red, powder, suitable for cell culture	description With 15 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture	description With L-alanyl-L-glutamine, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
sterility sterile-filtered	sterility -	sterility sterile-filtered	sterility sterile-filtered
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
form liquid	form powder	form liquid	form liquid
components L-glutamine: no phenol red: no NaHCO₃: yes HEPES: 15 mM sodium pyruvate: 0.055 g/L glucose: 3.15 g/L	components NaHCO₃: no L-glutamine: yes phenol red: no sodium pyruvate: 0.055 g/L glucose: 3.15 g/L HEPES: 15 mM	components sodium pyruvate: 0.055 g/L L-glutamine: no phenol red: yes NaHCO₃: yes HEPES: 15 mM glucose: 3.15 g/L	components phenol red: yes stable glutamine: yes glucose: high HEPES: no NaHCO₃: yes sodium pyruvate: yes
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient

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Pictogrammes

GHS07

Mot-clé

Warning

Codes de danger

H317

Mentions de précaution

P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364

Hazard Classifications

Skin Sens. 1

Classe de stockage

12 - Non Combustible Liquids

Que se passe-t-il ?

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)