OGS93

PSF-CMV-NH2-HA-EKT-NCOI - N-TERMINAL FLU HA TAG PLASMID

plasmid vector for molecular cloning

• Synonyme(s) : cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
• NACRES: NA.85
• UNSPSC Code: 12352200
• Promoter: Promoter name: CMV; Promoter activity: constitutive; Promoter type: mammalian
• Origin of replication: pUC (500 copies)
• Bacteria selection: kanamycin
• Reporter gene: none
• Peptide cleavage: no cleavage

Propriétés

• tag: Hemagglutinin A (HA) tagged
• form: buffered aqueous solution
• mol wt: size 4265 bp
• bacteria selection: kanamycin
• origin of replication: pUC (500 copies)
• peptide cleavage: no cleavage
• peptide tag location: N-terminal
• promoter: Promoter name: CMV; Promoter activity: constitutive; Promoter type: mammalian
• reporter gene: none
• shipped in: ambient
• storage temp.: −20°C

Description

General description

This plasmid adds a HA epitope tag to the N-terminus of a protein that is encoded within the multiple cloning site. This tag allows the detection and purification of a tagged protein using antibodies raised against the Influenza HA epitope. The HA tag coding sequence is YPYDVPDYA. There is an enterokinase cleavage site (DDDDK) immediately downstream of the HA tag that can be used to remove the HA tag from a  purified protein. It cleaves after the lysine residue.

Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.

Application

Cloning in a gene: This vector has been designed to allow the addition of a peptide tag to the end of a protein of interest using standard cloning techniques.Multiple Cloning Site Notes:

There is a start codon in the NcoI site can be removed by digestion with KpnI if required. The MCS for gene insertions extends from NotI to XbaI however the tag resides between the NotI and HindIII sites. There are Shine-Dalgarno sequences and KOZAK sequences aligned with the start codon of the peptide tag.

The ClaI to NheI sites have other functions such as adding C-terminal peptide tags second promoters or IRES expression components. The BsgI and BseRI restriction sites cleave within the stop codon in the XbaI site and allow the retrospective fusion of C-terminal peptide tags sequences if the stop codon is placed in this position.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.oxgene.com

Other Notes

To view sequence information for this product, please visit the product page

Informations sur la sécurité

• Classe de stockage: 12 - Non Combustible Liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable