HPLC Analysis of Fluoroacetate and Fluoride on SeQuant^® ZIC-HILIC with Radiometric Detection

column

Sequant ZIC-HILIC, PEEK, 250 x 4.6 mm, 5 μm, 200 Å (1.50458.0001)

mobile phase

[A] 50 mM ammonium hydrogen carbonate; [B] acetonitrile

gradient

10% A for 3 min; to 80% A in 0.5 min; held at 80% A for 5.5 min; to 10% A in 0.5 min

column temp.

ambient

flow rate

2 mL/min

detector

Bioscan 3000 radiodetector

injection

50 μL