HPLC Analysis of Spironolactone on Chromolith^® HighResolution RP-18 endcapped 100 x 4.6 mm

column

Chromolith^® HighResolution RP-18 endcapped, 100 x 4.6 mm (1.52022.0001)

mobile phase

[A] water; [B] methanol; (40:60, A:B)

flow rate

1.5 mL/min

pressure

1175 psi (81 bar)

column temp.

ambient

detector

230 nm (cell: 11 μL)

injection

13 μL (reduced with 33% as it is scaled to a 33% shorter column with same diameter)

sample

0.5 mg/mL (500 ppm) of Spironolactone RS in diluent (diluent: acetonitrile/water 1:1 (v/v))