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648317

トリズマ®塩酸塩

Useful in the pH range of 7.0-9.0. Has a pKa of 8.1 at 25°C.

別名:

Trizma® 塩酸塩, TRIS HCl, TRIS 塩酸塩, トリス(ヒドロキシメチル)アミノメタン 塩酸塩, トロメタン塩酸塩

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この商品について

化学式:
NH2C(CH2OH)3 · HCl
CAS番号:
分子量:
157.60
NACRES:
NA.25
UNSPSC Code:
12161705
Beilstein/REAXYS Number:
3675235
MDL number:
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grade

Molecular Biology

Quality Segment

description

RTECS - TY2900000

assay

≥99% (titration)

form

crystalline solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, desiccated (hygroscopic)

color

white

useful pH range

7.0-9.0

pKa (25 °C)

8.1

solubility

water: soluble

cation traces

heavy metals (as Pb): ≤0.0002%

absorption

≤0.05 at 260 nm, ≤0.05 at 280 nm

foreign activity

DNases, proteases, RNases, none detected

shipped in

ambient

storage temp.

15-25°C

SMILES string

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

InChI key

QKNYBSVHEMOAJP-UHFFFAOYSA-N

General description

Useful in the pH range of 7.0-9.0. Has a pKa of 8.1 at 25°C.
Widely used biological buffer (absorbance: ≤0.05 at 260 nm and 280 nm). Useful in the pH range of 7.0-9.0. Has a pKa of 8.1 at 25°C.

Other Notes

すべてのバッファーのpHは、温度や濃度に依存します。トリスバッファーの場合、温度が1°C低下するとpHは約0.03上昇し、希釈10倍ごとにpHは0.03~0.05低下します。精密なアプリケーションには、適切に校正した、ガラス/カロメル複合電極のpHメーターを使用してください。

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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当該品目
10812846001PHR9293108319
description

Useful in the pH range of 7.0-9.0. Has a pKa of 8.1 at 25°C.

description

>99% (titration), pH 7.0-9.0, suitable for FISH

description

certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

description

EMPROVE® EVOLVE

assay

≥99% (titration)

assay

>99% (titration)

assay

-

assay

-

grade

Molecular Biology

grade

-

grade

certified reference material, TraceCERT®

grade

-

Quality Level

100

Quality Level

-

Quality Level

300

Quality Level

400

form

crystalline solid

form

solid

form

-

form

powder

storage condition

OK to freeze, desiccated (hygroscopic)

storage condition

-

storage condition

-

storage condition

-

storage temp.

15-25°C

storage temp.

room temp

storage temp.

2-8°C

storage temp.

-


保管分類

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

648317-100RGM: + 648317-100GM: + 648317-1KG: + 648317-1RKG: + US2648317-1KG: + US2648317-100GM-B:

jan



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質問

1–3/3 質問  
  1. How can i prepare the buffer HCL solution?

    1 回答
    1. An amount of this product would need to be added to water in order to create the concentration of the desired buffer (e.g. 157.60 grams for one liter of a 1 M solution). The pH can then be adjusted as necessary.

      役に立ちましたか?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 回答
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      役に立ちましたか?

  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 回答
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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