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manufacturer/tradename
BPS Bioscience
technique(s)
activity assay: suitable (Fluorogenic)
UniProt accession no.
storage temp.
-65 to -96°C
General description
The NAMPT Inhibitor Screening Assay Kit is designed to measure NAMPT activity for screening and profiling applications. The NAMPT assay kit comes in a convenient 96-well format, with purified recombinant NAMPT enzyme, NAMPT assay buffer, NAMPT dilution buffer, ATP, Nicotinamide, PRPP, and Ethanol sufficient for 96 enzyme reactions. The key to the assay is a coupled reaction including NMNAT and ADH. NAMPT converts nicotinamide substrate to nicotinamide mononucleotide (NMN) via PRPP. NMNAT subsequently converts NMN to NAD+ , which is reduced by ADH to NADH, permitting detection of the fluorescent NADH signal.
Application
Study enzyme kinetics and screen small molecule inhibitors for drug discovery and High Throughput Screen (HTS) applications.
Nicotinamide phosphoribosyltransferase (NAMPT) catalyzes the formation of nicotinamide mononucleotide (NMN) from nicotinamide and 5-phosphoribosyl-1-pyrophosphate (PRPP). It is the rate limiting enzyme in the mammalian NAD (nicotinamide adenine dinucleotide) biosynthesis pathway. NAMPT is thought to be involved in many important biological processes, including metabolism, stress response, and aging. NAMPT is often overexpressed in cancer cells, an adaptation required to sustain the higher energetic need of the cells. In addition, it also plays roles in proliferation, invasion, stemness, angiogenesis, immune regulation, and drug resistance of tumors, making it an attractive target candidate for cancer therapy.
Datasheet and Protocol for 96 reaction kit may be found here.
Datasheet and Protocol for 384 reaction kit may be found here.
Nicotinamide phosphoribosyltransferase (NAMPT) catalyzes the formation of nicotinamide mononucleotide (NMN) from nicotinamide and 5-phosphoribosyl-1-pyrophosphate (PRPP). It is the rate limiting enzyme in the mammalian NAD (nicotinamide adenine dinucleotide) biosynthesis pathway. NAMPT is thought to be involved in many important biological processes, including metabolism, stress response, and aging. NAMPT is often overexpressed in cancer cells, an adaptation required to sustain the higher energetic need of the cells. In addition, it also plays roles in proliferation, invasion, stemness, angiogenesis, immune regulation, and drug resistance of tumors, making it an attractive target candidate for cancer therapy.
Datasheet and Protocol for 96 reaction kit may be found here.
Datasheet and Protocol for 384 reaction kit may be found here.
注意喚起語
Warning
ハザードコード
Hazard Classifications
Flam. Liq. 3
保管分類
3 - Flammable liquids
一体何が起きているの？
WGK 1
適用法令
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Please refer to KIT Component information
pdsc
Please refer to KIT Component information
prtr
Please refer to KIT Component information
fsl
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ishl_indicated
Please refer to KIT Component information
ishl_notified
Please refer to KIT Component information
cart
キットコンポーネントの情報を参照してください
jan
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| BPS71276-1 | 04069876045009 |
| BPS71276-2 | 04069876045016 |