The Product Information Sheet for H9268 advises, "Stock solutions in 0.9% NaCl at 1 mg/mL remain active at least one year stored at 2–8°C." There is no data indicating the stability of a solution in DPBS stored at -80°C.
H9268
臭化ヘキサジメトリン
≥94% (titration)
別名:
1,5-ジメチル-1,5-ジアザウンデカメチレンポリメトブロミド, ポリブレン
スキップする
1 of 4
当該品目 | |||
|---|---|---|---|
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. - |
| assay ≥94% (titration) | assay - | assay ≥95% | assay - |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
General description
ヘキサジメトリンブロミド（臭化ヘキサジメトリン）は、ヘパリンアンタゴニストとして働く四級アンモニウム化合物です。これは、低分子量のDNAの形質転換にも関与しています。
Application
ヘキサジメトリンブロミド（臭化ヘキサジメトリン）を用いて哺乳動物細胞にDNAをトランスフェクトできます。これを使用して、リポフェクショントランスフェクションの効率を高めることができます。
ヘキサジメトリンブロミド（臭化ヘキサジメトリン）は、細胞内のレンチウイルス感染に使用されています。これは、レトロウイルス上清による細胞の感染に使用されています。
ヘキサジメトリンブロミド（臭化ヘキサジメトリン）は、細胞内のレンチウイルス感染に使用されています。これは、レトロウイルス上清による細胞の感染に使用されています。
Related product
製品番号
詳細
価格
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral
保管分類
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
H9268-10G: + H9268-50G: + H9268-100G: + H9268-BULK: + H9268-VAR: + H9268-5G:
jan
Redei GP
Encyclopedia of Genetics, Genomics, Proteomics, and Informatics (2008)
E2F1 modulates p38 MAPK phosphorylation via transcriptional regulation of ASK1 and Wip1.
Hershko T
The Journal of Biological Chemistry, 281, 31309-31309 (2006)
UCHL1 Is a Putative Tumor Suppressor in Ovarian Cancer Cells and Contributes to Cisplatin Resistance.
Jin C et al.
Journal of Cancer, 4, 662-662 (2013)
Blood (2013)
Gaetana Restivo et al.
Nature communications, 8(1), 1988-1988 (2017-12-08)
Cutaneous melanoma represents the most fatal skin cancer due to its high metastatic capacity. According to the "phenotype switching" model, the aggressive nature of melanoma cells results from their intrinsic potential to dynamically switch from a high-proliferative/low-invasive to a low-proliferative/high-invasive
資料
Small interfering RNAs (siRNAs) are powerful tools for gene expression knockdown, widely used in molecular biology.
Our lentiviral vector systems are developed with enhanced safety features. Numerous precautions are in place in the design of our lentiviruses to prevent replication. Good handling practices are a must.
You are not alone designing successful CRISPR, RNAi, and ORF experiments. Sigma-Aldrich was the first company to commercially offer lentivirus versions of targeted genome modification technologies and has the expertise and commitment to support new generations of scientists.
The introduction of small interfering RNAs (siRNAs) into cultured cells provides a fast and efficient means of knocking down gene expression and has allowed siRNAs to quickly become a ubiquitous tool in molecular biology.
プロトコル
Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic change.
Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.
FACS sorts cells based on light scattering and fluorescence for objective cell analysis.
安定で長期的な遺伝子サイレンシングおよび表現型の変化を達成するための、MISSION shRNAレンチウイルス粒子の形質導入（トランスダクション）の詳細な手順
-
After reconstituting H9268 in DPBS, aliquoting and storing at -80C, how long is the solution good for?
1 回答-
役に立ちましたか？
-
-
How can I determine the shelf life / expiration / retest date of this product?
1 回答-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf役に立ちましたか？
-
-
Is it possible for hexadimethrine bromide to breakdown and lose its effectiveness after 3 years when stored in its powdered form at 4 degree celsius? We bought this product around 3 years and now it doesn't work as well as before when doing transduction.
1 回答-
This product is assigned a recommended retest period of 2 years from the date of Quality release. While quite stable, the compound may slowly degrade over time. The suitability of this item beyond the lot specific retest date would have to be determined by the end user.
役に立ちましたか？
-
-
How is shipping temperature determined? And how is it related to the product storage temperature?
1 回答-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
役に立ちましたか？
-
-
what is the concentration of 5G polybrene? Does G stands for gram?
1 回答-
This product is in powder form and not a prepared solution. The minimum purity of this material is 94%. The 'G' represents grams.
役に立ちましたか？
-
-
What is the preferred solvent for Hexadimethrine bromide? I see water or saline above, but does either effect solubility or stability?
1 回答-
The choice of solvent typically depends on the specific application. For in vitro or in vivo studies, saline is more commonly used. The material is soluble in water at up to 10%. Solutions are quite stable and maintain activity after autoclaving. Stock solutions prepared at 1 mg/mL in saline are stable for up to 1 year when stored at 2–8 °C. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/253/063/h9268bul.pdf役に立ちましたか？
-
-
How long is the Hexadimethrine Bromide powder stable? I cannot find its expiry date.
1 回答-
This product in powdered format is stable for up to a recommended retest period of two years as listed on the Certificate of Analysis. After the Retest Date, product samples should be examined to ensure that the product is still in compliance with the established specifications.
When in solution, this product will remain active for at least one year if stored at 2–8 °C. For more information please view the product data sheet: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/253/063/h9268bul.pdf
役に立ちましたか？
-
-
How do I sterilize Product H9268, Hexadimethrine bromide?
1 回答-
Hexadimethrine bromide can be filtered sterilized or autoclaved.
役に立ちましたか？
-
-
What is the solution stability of Product H9268, Hexadimethrine bromide?
1 回答-
Stock solutions in 0.9% saline solution at 1 mg/mL are stable at least one year stored in the refrigerator. Stability information is found on the Product Bulletin.
役に立ちましたか？
-
-
What is the Department of Transportation shipping information for this product?
1 回答-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
役に立ちましたか？
-
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）