P4591
ピルビン酸オキシダーゼ 細菌
lyophilized powder, ≥1.5 U/mg
別名:
ピルビン酸:酸素オキシドレダクターゼ(リン酸化)
ログインで組織・契約価格をご覧ください。
サイズを選択してください
表示を変更する
| サイズ/SKU | 在庫状況 | 単価 |
|---|---|---|
100 units | カートの在庫状況を確認する | ￥46,800 |
この商品について
CAS番号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-644-5
MDL number:
EC Number:
Specific activity:
≥1.5 U/mg
￥46,800
カートの在庫状況を確認する
General description
Isoelectric point : 4.3
Michaelis constant : 3.4 X 10-4M (Pyruvate)
Inhibitors : Fe++,Zn++,Cu++,Ag+,Hg++
Optimum pH : 5.7
Optimum temperature : 65oC
pH Stability : pH 5.7 - 6.5 (25oC, 20hr)
Thermal stability : below 45oC (pH 6.0, 15min)
Michaelis constant : 3.4 X 10-4M (Pyruvate)
Inhibitors : Fe++,Zn++,Cu++,Ag+,Hg++
Optimum pH : 5.7
Optimum temperature : 65oC
pH Stability : pH 5.7 - 6.5 (25oC, 20hr)
Thermal stability : below 45oC (pH 6.0, 15min)
Application
Pyruvate Oxidase from microorganisms has been used in the Amplex Red-based fluorescence assay for pyruvate. It has also been used in developing near real-time continuous detection system for pyruvate.
Pyruvate Oxidase (PoxB) converts pyruvate directly to acetate and CO2. It is used to study pyruvate metabolism. It is used to study aerobic metabolism of bacterium, such as Lactobacillus plantarumand Streptococcus pneumoniae. Pyruvate Oxidase is used for enzymatic determination of pyruvate, GOT, and GPT in clinical analysis.
Biochem/physiol Actions
Pyruvate Oxidase oxidises pyruvate to form acetate, hydrogen peroxide and carbondioxide. Pyruvate oxidase requires flavin adenine dinucleotide (FAD), thiamine pyrophosphate (TPP) and magnesium as cofactors for its catalytic activity. Pyruvate oxidase is activated by the cofactor thiamine.
Pyruvate Oxidase consists of four subunits with identical molecular weights. PoxB reacts with certain aldehydes and phosphate can be replaced by arsenate. Oxygen as well as several artificial compounds can function as electron acceptors. Pyruvate Oxidase is activated by phospholipids as well as monomeric and micellar amphiphiles.
Physical form
凍結乾燥品(安定剤としてFADと糖を含有)
Other Notes
1ユニットは、pH 5.7、37°Cでピルビン酸とリン酸がアセチルリン酸とCO2へと変換される反応において、1分間で、1.0 μmolのH2O2を生成する酵素量です。
1 of 1
当該品目 | |||
|---|---|---|---|
| description lyophilized powder, ≥1.5 U/mg | description lyophilized powder, ≥50 units/mg protein, recombinant, expressed in E. coli | description lyophilized powder, ≥35 units/mg protein (biuret) | description aqueous solution, ≥30 units/mg protein (biuret) |
| specific activity ≥1.5 U/mg | specific activity ≥50 units/mg protein | specific activity ≥35 units/mg protein (biuret) | specific activity ≥30 units/mg protein (biuret) |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form aqueous solution |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −70°C |
| mol wt ~260 kDa | mol wt 64 kDa | mol wt - | mol wt 62 kDa |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
注意喚起語
Danger
ハザードコード
Hazard Classifications
Resp. Sens. 1
保管分類
11 - Combustible Solids
一体何が起きているの？
WGK 3
引火点 (F)
Not applicable
引火点（°C）
Not applicable
PPE（個人用保護具）
Eyeshields, Gloves, type N95 (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
P4591-BULK: + P4591-VAR: + P4591-100UN: + P4591-25UN: + P4591-10UN:
jan