Merck
All Photos(1)

D6546

Sigma-Aldrich

Medio de Eagle modificado de Dulbecco, glucosa elevada

With 4500 mg/L glucose, sodium pyruvate, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

All Photos(1)
Synonym(s):
DME, DMEM
NACRES:
NA.75

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: yes
phenol red: yes
sodium pyruvate: yes
HEPES: no
L-glutamine: no
glucose: high

shipped in

ambient

storage temp.

2-8°C

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This Item
FG0435D5796D6429
Sigma-Aldrich

Sigma-Aldrich

D6546

Dulbecco′s Modified Eagle′s Medium - high glucose

Sigma-Aldrich

Sigma-Aldrich

FG0435

Dulbecco′s Modified Eagle′s Medium - high glucose

Dulbecco’s Modified Eagle’s Medium - high glucose With 4500 mg/L glucose, L-glutamine, and sodium bicarbonate, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

D5796

Dulbecco′s Modified Eagle′s Medium - high glucose

Sigma-Aldrich

Sigma-Aldrich

D6429

Dulbecco′s Modified Eagle′s Medium - high glucose

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

form

liquid

form

liquid

form

liquid

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

impurities

-

impurities

endotoxin, tested

impurities

endotoxin, tested

components

NaHCO3: yes
phenol red: yes
sodium pyruvate: yes
HEPES: no
L-glutamine: no
glucose: high

components

phenol red: yes
glucose: high
stable glutamine: yes
sodium pyruvate: no
HEPES: no
NaHCO3: yes

components

NaHCO3: yes
L-glutamine: yes
glucose: high
phenol red: yes
sodium pyruvate: no
HEPES: no

components

HEPES: no
NaHCO3: yes
phenol red: yes
sodium pyruvate: yes
L-glutamine: yes
glucose: high

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

General description

Este medio de glucosa DMEM-Hi es un medio completo 1x con piruvato sódico añadido. También difiere de la formulación DMEM-Hi original en que se sustituye la piridoxina por el piridoxal. El piridoxal es un componente inestable de los medios. Este medio requiere enriquecimiento con L-glutamina o L-alanil-L-glutamina.

Application

El medio de Eagle modificado de Dulbecco (DMEM) es una modificación del medio basal Eagle (BME) que contiene el cuádruple de concentraciones de aminoácidos y vitaminas. La formulación original contenía 1000 mg/l de glucosa y se utilizaba para cultivar células embrionarias de ratón. Desde entonces, se ha modificado de varias formas para soportar cultivos primarios de células de ratón y de pollo, así como una variedad de células normales y transformadas. Cada uno de estos medios ofrece una combinación diferente de L-glutamina y piruvato sódico. Además, los niveles de glucosa se han elevado a 4 500 mg/l, lo que contribuye al nombre «DMEM/High».

Reconstitution

Enriquecer con 0,584 g/l de L-glutamina.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317

Hazard Classifications

Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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