Merck
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D5796

Sigma-Aldrich

Medio de Eagle modificado de Dulbecco, glucosa elevada

With 4500 mg/L glucose, L-glutamine, and sodium bicarbonate, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

All Photos(1)
Synonym(s):
DME, DMEM
NACRES:
NA.75

Nivel de calidad

400

esterilidad

sterile-filtered

formulario

liquid

technique(s)

cell culture | mammalian: suitable

impurezas

endotoxin, tested

componentes

NaHCO3: yes
L-glutamine: yes
glucose: high
phenol red: yes
sodium pyruvate: no
HEPES: no

enviado en

ambient

temp. de almacenamiento

2-8°C

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This Item
D6546FG0435D6429
Dulbecco’s Modified Eagle’s Medium - high glucose With 4500 mg/L glucose, L-glutamine, and sodium bicarbonate, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

D5796

Dulbecco′s Modified Eagle′s Medium - high glucose

Sigma-Aldrich

Sigma-Aldrich

D6546

Dulbecco′s Modified Eagle′s Medium - high glucose

Sigma-Aldrich

Sigma-Aldrich

FG0435

Dulbecco′s Modified Eagle′s Medium - high glucose

Sigma-Aldrich

Sigma-Aldrich

D6429

Dulbecco′s Modified Eagle′s Medium - high glucose

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

form

liquid

form

liquid

form

liquid

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

-

impurities

endotoxin, tested

components

NaHCO3: yes
L-glutamine: yes
glucose: high
phenol red: yes
sodium pyruvate: no
HEPES: no

components

NaHCO3: yes
phenol red: yes
sodium pyruvate: yes
HEPES: no
L-glutamine: no
glucose: high

components

phenol red: yes
glucose: high
stable glutamine: yes
sodium pyruvate: no
HEPES: no
NaHCO3: yes

components

HEPES: no
NaHCO3: yes
phenol red: yes
sodium pyruvate: yes
L-glutamine: yes
glucose: high

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

Descripción general

Este medio de glucosa DMEM-Hi es un medio completo 1x sin factores añadidos (modificaciones comunes) como L-alanil-L-glutamina, HEPES o piruvato sódico. Difiere de la formulación DMEM-Hi original en que se sustituye la piridoxina por el piridoxal. El piridoxal es un componente inestable de los medios.

Aplicación

El medio de Eagle modificado de Dulbecco (DMEM) es una modificación del medio basal Eagle (BME) que contiene el cuádruple de concentraciones de aminoácidos y vitaminas. La formulación original contenía 1000 mg/l de glucosa y se utilizaba para cultivar células embrionarias de ratón. Desde entonces, se ha modificado de varias formas para soportar cultivos primarios de células de ratón y de pollo, así como una variedad de células normales y transformadas. Cada uno de estos medios ofrece una combinación diferente de L-glutamina y piruvato sódico. Además, los niveles de glucosa se han elevado a 4 500 mg/l, lo que contribuye al nombre «DMEM/High».

Código de clase de almacenamiento

12 - Non Combustible Liquids

WGK

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

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