L3771

Sodium dodecyl sulfate

BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC)

• Synonym(s): Dodecyl sodium sulfate, Dodecyl sulfate sodium salt, Lauryl sulfate sodium salt, SDS, Sodium lauryl sulfate
• Linear Formula: CH₃(CH₂)₁₁OSO₃Na
• CAS Number: 151-21-3
• Molecular Weight: 288.38
• Beilstein: 3599286
• Número de EC: 205-788-1
• Número MDL: MFCD00036175
• eCl@ss: 39093306
• ID de la sustancia en PubChem: 24896351
• NACRES: NB.22

Properties

• grado: for molecular biology
• Nivel de calidad: 200
• descripción: anionic
• Línea del producto: BioReagent
• ensayo: ≥98.5% (GC)
• mol peso: micellar 18,000
• número de agregación: 62
• technique(s): electrophoresis: suitable
• CMC: 7-10 mM (20-25°C)
• mp: 204-207 °C (lit.)
• temperatura de transición: cloud point >100 °C
• solubilidad: water: soluble
• trazas de anión: chloride (Cl^-): ≤500 ppm; phosphate (PO₄^3-): ≤10 ppm
• trazas de catión: heavy metals (as Pb): ≤10 ppm
• absorción: ≤0.1 at 260 at 3%; ≤0.1 at 280 at 3%
• HLB: 40
• idoneidad: suitable for electrophoresis
• actividad extraña: DNase, RNase, none detected
• SMILES string: [Na+].CCCCCCCCCCCCOS([O-])(=O)=O
• InChI: 1S/C12H26O4S.Na/c1-2-3-4-5-6-7-8-9-10-11-12-16-17(13,14)15;/h2-12H2,1H3,(H,13,14,15);/q;+1/p-1
• InChI key: DBMJMQXJHONAFJ-UHFFFAOYSA-M

Description

Descripción general

Sodium dodecyl sulfate (SDS) is an anionic detergent suitable to denature proteins. It has a long-chain aliphatic group with negatively charged sulfate attached. This makes SDS an amphipathic detergent.

Aplicación

Sodium dodecyl sulfate has been used:

• in chromatin immunoprecipitation
• in SDS-polyacrylamide gel electrophoresis (SDS-PAGE)
• as a component of radioimmunoprecipitation assay buffer for immunoblotting

Used to solubilize and denature proteins for denaturing-PAGE. Most proteins bind SDS in a ratio of 1.4 g SDS per gram of protein. The charges intrinsic to the protein become insignificant compared to the overall negative charge provided by the bound SDS. The charge to mass ratio is essentially the same for each protein and will migrate in the gel based only on their size.

Anionic detergent

Envase

25, 100, 500 g in poly bottle

1, 10 kg

Nota de análisis

Tested for use in denatured polyacrylamide gel electrophoresis.

Safety Information

• pictogramas: GHS02,GHS05,GHS07
• Palabra de señalización: Danger
• Frases de peligro: H228 - H302 + H332 - H315 - H318 - H335 - H412
• Consejos de prudencia: P210 - P273 - P280 - P301 + P312 - P304 + P340 + P312 - P305 + P351 + P338
• Clasificaciones de peligro: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Flam. Sol. 2 - Skin Irrit. 2 - STOT SE 3
• Órganos de actuación: Respiratory system
• Código de clase de almacenamiento: 4.1B - Flammable solid hazardous materials
• WGK: WGK 2
• Punto de inflamabilidad F: 338.0 °F
• Punto de inflamabilidad C: 170 °C
• Equipo de protección personal: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges