No, bacteria are below the size threshold for counting on the Scepter. Sensor tips are designed to be single use.
PHCC340KIT
Scepter 3.0 Handheld Automated Cell Counter
Rapid cell counts in a handheld easy to use format, includes pkg of 40 μm Scepter 3.0 Cell Counter Sensors
Scepter 3.0 Handheld Automated Cell Counter
Synonym(s):
Cell counting instrument
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About This Item
UNSPSC Code:
41131501
NACRES:
NC.56
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description
Rapid cell counts in a handheld easy to use format, includes pkg of 40 μm Scepter 3.0 Cell Counter Sensors
manufacturer/tradename
Scepter
technique(s)
cell counting: suitable
particle size
4-18 μm
compatibility
for use with cells sized 4.0–25.0 μm
shipped in
ambient
1 of 4
This Item | PHCC360KIT | PHCC360500 | PHCC340050 |
|---|---|---|---|
| compatibility for use with cells sized 4.0–25.0 μm | compatibility for use with cells sized 4.0–25.0 μm | compatibility Scepter for use with cells sized 8.0–25.0 μm | compatibility Scepter for use with cells sized 5.0–15.0 μm |
| technique(s) cell counting: suitable | technique(s) cell counting: suitable | technique(s) cell counting: suitable | technique(s) cell counting: suitable |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| description Rapid cell counts in a handheld easy to use format, includes pkg of 40 μm Scepter 3.0 Cell Counter Sensors | description - | description - | description - |
| manufacturer/tradename Scepter | manufacturer/tradename Scepter | manufacturer/tradename Scepter | manufacturer/tradename Scepter |
| particle size 4-18 μm | particle size 6-36 μm | particle size 6-36 μm | particle size 4-18 μm |
General description
The Scepter 3.0 Handheld Automated Cell Counter delivers rapid cell counts in a handheld, easy to use format. Intended for research use only, Scepter 3.0 has been tested with cell types representative of those in use today. After preparing a dilution of the cell culture of interest, on-screen instructions guide the user through the sampling process. In less than 30 seconds after the Scepter Cell Counter aspirates a sample into the Scepter Cell Counter Sensor, the Scepter cell counter′s screen displays:
The Scepter 3.0 Cell Counter is compatible with both the 40uM and 60uM Scepter 3.0 Cell Counter Sensors. This kit (PHCC340KIT) includes a free 50-pack of 40uM sensors.
- Cell concentration
- Average cell size
- Average cell volume
- Histogram of size or volume distribution
The Scepter 3.0 Cell Counter is compatible with both the 40uM and 60uM Scepter 3.0 Cell Counter Sensors. This kit (PHCC340KIT) includes a free 50-pack of 40uM sensors.
Other Notes
- 1 Scepter 3.0 Automated Handheld Cell Counter
- 1 Test Bead Vial
- 1 Charging Station
- 50 Sensors (40μM)
The Scepter 3.0 Cell Counter is not compatible with previous generation sensors; only compatible with Scepter 3.0 Cell Counter Sensors shown in the Related Products.
accessory
Product No.
Description
Pricing
Storage Class Code
10-13 - German Storage Class 10 to 13
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Articles
Use of MULTI-seq lipid-modified oligos, protocol, and troubleshooting guide for PCR Assays and Sequencing applications.
Cell counting protocol using a hemocytometer for monitoring cell viability. Discover other cell counting tools such as the Scepter™ 3.0 Cell Counter.
The Scepter™ 3.0 Cell Counter, when used in conjunction with a 40 μm aperture sensor, can accurately count and precisely size a wide range of cell types and particles. This application note demonstrates two examples of experiments using the instrument’s sensitive size-discriminating capacity for rapid qualitative assessment of individual cell populations.
Protocols
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Learn how to perform cell migration assays in vitro using Millicell® hanging cell culture inserts and the suspension T-cell lines Jurkat and primary CD4+ cells. Monitor migration by flow cytometry and EZ-MTT assays.
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Scepter™ counting is 7 to 10 times faster than hemocytometry-and also faster than other automated counters. Learn more about how this amazing technology works
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Will Scepter 3.0 be able to quantify bacterial cultures? How many reads is it possible with a single counter sensor?
1 answer-
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Can it count nuclei, specifically isolated from human aortic endothelial cells?
1 answer-
While most mammalian nuclei are around 6 micrometers (um), which is at the low end of detection, this size range can also include other cell debris that is often gated out during analysis. Additionally, since the counts are displayed as a normal distribution, the full profile may not be visible at the low end of detection. Generally, it's not recommended to use this method for small nuclei, but the Scepter may work for some cells with larger nuclei.
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