MAB1083

Anti-GFP Antibody, clone 3F8.2

clone 3F8.2, from mouse

• Synonim(y): Green Fluorescent protein
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• białko sprzężone: unconjugated
• forma przeciwciała: purified immunoglobulin
• rodzaj przeciwciała: primary antibodies
• klon: 3F8.2, monoclonal
• reaktywność gatunkowa: vertebrates
• metody: western blot: suitable
• izotyp: IgG1κ
• numer dostępu UniProt: P42212
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: unmodified

Opis

Opis ogólny

28 kDa

The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.

Immunogen

Epitope: Full Length

Full length GFP protein.

Zastosowanie

Detect GFP using this Anti-GFP Antibody, clone 3F8.2 validated for use in WB.

Research Category
Secondary & Control Antibodies

Research Sub Category
Epitope Tags

Western Blot Analysis:
0.125 μg/mL - 1 μg/mL of this antibody detected GFP in 10 ng of GFP recombinant protein (Cat no. 14-392).

Działania biochem./fizjol.

Reacts with most common vertebrate proteins containing the GFP tag.

The antibody reacts with the 28 kDa GFP protein.

Postać fizyczna

Format: Purified

Protein G Purified

Purified mouse immunoglobulin in buffer containing PBS containing 0.05% sodium azide.

Uwaga dotycząca przygotowania

Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Komentarz do analizy

Control
Western Blot: GFP recombinant protein

Routinely evaluated by Western Blot on GFP recombinant protein.

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable