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P5381

Phosphoglucose Isomerase from baker′s yeast (S. cerevisiae)

Type III, ammonium sulfate suspension, ≥400 units/mg protein (biuret)

Synonim(y):

D-Glucose-6-phosphate ketol-isomerase, PGI, Phosphosaccharomutase

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Rozmiar/SKUDostępnośćCena netto
1000 units
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643,00 zł
5000 units
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
1950,00 zł
50000 units
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
12 570,00 zł

Informacje o tej pozycji

Numer CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-603-1
MDL number:
Numer WE:
eCl@ss:
32160701
Specific activity:
≥400 units/mg protein (biuret)
Biological source:
bakers yeast
Concentration:
≥3 mg/mL protein Biuret

643,00 zł


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biological source

bakers yeast

Quality Segment

type

Type III

form

ammonium sulfate suspension

specific activity

≥400 units/mg protein (biuret)

mol wt

tetramer 119,500 Da±600

concentration

≥3 mg/mL protein Biuret

technique(s)

activity assay: suitable

color

white

Protein ID accession no.

UniProt accession no.

storage temp.

2-8°C

Gene Information

yeast ... PGI1(852495)

General description

Research area: Cell Signaling

Phosphoglucose Isomerase ubiquitous multifunctional cytosolic protein. This dimeric protein has a molecular weight of approximately 119,500 ± 600 and is composed of four subunits that are nearly identical.

Application

Phosphoglucose Isomerase from baker′s yeast (S. cerevisiae) has been used:
  • to convert fructose-6-phosphate to glucose-6-phosphate during the enzymatic measurement of serum mannose levels.
  • as one of the enzymes in the series of four enzyme-coupled reactions to measure phosphomannomutase (PMM) activity.
  • to convert fructose to glucose while evaluating fructose content in kudzu roots.

Biochem/physiol Actions

Phosphoglucose Isomerase(PGI) is an enzyme crucial for the interconversion of D-glucose 6-phosphate and D-fructose 6-phosphate. PGI is responsible for the second step of glycolysis and is involved in glucogenesis and pentose phosphate pathway (PPP). It is highly conserved in bacteria and eukaryotes. PGI plays a vital role in the survival of A. fumigatus and demonstrates diverse functions that encompass development, biosynthesis of cell wall glucan, and virulence.Phosphoglucose Isomerase functions as an isomerase, neuroleukin, autocrine motility factor, and a differentiation and maturation mediator.

Physical form

Crystalline suspension in 2.6 M (NH4)2SO4 containing 0.1 mM magnesium acetate, pH 7.0

Other Notes

One unit will convert 1.0 μmole of D-fructose 6-phosphate to D-glucose 6-phosphate per min at pH 7.4 at 25 °C.
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Ta pozycja
F2668T2507P7634
description

Type III, ammonium sulfate suspension, ≥400 units/mg protein (biuret)

description

for use with Fructose Assay Kit FA-20

description

Type I, ammonium sulfate suspension, ~10,000 units/mg protein

description

ammonium sulfate suspension, ≥500 units/mg protein

technique(s)

activity assay: suitable

technique(s)

photometry: suitable

technique(s)

-

technique(s)

-

Gene Information

yeast ... PGI1(852495)

Gene Information

-

Gene Information

-

Gene Information

-

specific activity

≥400 units/mg protein (biuret)

specific activity

-

specific activity

~10,000 units/mg protein

specific activity

≥500 units/mg protein

biological source

bakers yeast

biological source

-

biological source

-

biological source

bakers yeast

form

ammonium sulfate suspension

form

-

form

ammonium sulfate suspension

form

ammonium sulfate suspension

concentration

≥3 mg/mL protein Biuret

concentration

-

concentration

-

concentration

1.0-10.0 mg/mL


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Piktogramy

Health hazard

Słowo ostrzegawcze

Danger

Kody zagrożeń

Ostrzeżenia

Hazard Classifications

Resp. Sens. 1

Klasa składowania

10 - Combustible liquids

WGK

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

PPE (środki ochrony indywidualnej)

Eyeshields, Gloves



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Certyfikaty analizy (CoA)

Lot/Batch Number

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Questions

1–5 of 5 Questions  
  1. What is the pH optimum of Phosphoglucose Isomerase from baker's yeast (e.g., Product No. P5381)?

    1 answer
    1. The pH optimum for yeast PGI (Product No. P5381) is 7, according to Marchand, M. et al., Eur. J. Biochem., 184, 455-464 (1989).

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  2. What is the substrate for Phosphoglucose Isomerase from baker's yeast (e.g., Product No. P5381)?

    1 answer
    1. The substrate for PGI is D-fructose-6-phosphate, according to our Enzyme Assay procedure.

      Helpful?

  3. What is the typical protein concentration for Product No. P5381 (PGI)?

    1 answer
    1. The protein concentration of Product No. P5381 (PGI) can vary from lot-to-lot. It is typically in the range of 5-20 mg protein per mL. The lot-specific concentration will be given on the certificate of analysis.

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  4. What is the KM value for yeast PGI (Product No. P5381) with D-Fructose-6-phosphate as substrate, and what does that mean?

    1 answer
    1. KM is the concentration of substrate that leads to half-maximal velocity. The KM value for yeast PGI (Product No. P5381) with D-Fructose-6-phosphate as substrate is 0.167, according to Marchand, M. et al., Eur. J. Biochem., 184, 455-464 (1989).

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  5. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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