Each vial content 20 ug of the product.
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T6567
Trypsin Protease
Hydrolyzes peptide bonds specifically at the carboxyl side of arginine and lysine residues, suitable for mass spectrometry, from Porcine pancreas
Synonim(y):
Porcine Trypsin, Trypsin for Mass Spectropetry
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| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
20 μg | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 285,00 zł |
5 x 20 μg | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 1020,00 zł |
1 mg | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 3640,00 zł |
Informacje o tej pozycji
Biological source:
Porcine pancreas
285,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócNazwa produktu
Trypsin from porcine pancreas, BioReagent, Proteomics Grade, Dimethylated
biological source
Porcine pancreas
Quality Segment
product line
BioReagent
technique(s)
protein mass spectrometry: suitable
solubility
1 mM HCl: soluble 1 mg/mL, clear, colorless
shipped in
wet ice
storage temp.
2-8°C
General description
Trypsin is a serine protease that is usually used in biochemistry and biology as an important enzymatic reagent. This method produces a highly purified trypsin product suitable for proteomics research. Proteomics Grade ideal for use in both solution and in-gel tryptic digestions. Trypsin, a serine protease, is present in the digestive system of several vertebrates.
Application
Trypsin from porcine pancreas is used for the following applications:
Biochem/physiol Actions
Trypsin is routinely used in proteomics research for peptide mapping and protein sequence work, due to its highly specific cleavage resulting in a limited number of tryptic peptides. It hydrolyzes peptide bonds specifically at the carboxyl side of arginine and lysine residues.. The enzyme also exhibits esterase and amidase activities. Trypsin acts as a cell culture tool. It is used to hydrolyze allergenic proteins to produce hypoallergenic milk in industries.
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Ta pozycja | |||
|---|---|---|---|
| description BioReagent, Proteomics Grade, Dimethylated | description Type IX-S, lyophilized powder, 13,000-20,000 BAEE units/mg protein | description ~1500 U/mg | description lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture, 1,000-2,000 BAEE units/mg solid |
| biological source Porcine pancreas | biological source - | biological source - | biological source Porcine pancreas |
| shipped in wet ice | shipped in - | shipped in - | shipped in wet ice |
| solubility 1 mM HCl: soluble 1 mg/mL, clear, colorless | solubility - | solubility - | solubility - |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
| product line BioReagent | product line - | product line - | product line BioXtra |
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Słowo ostrzegawcze
Danger
Kody zagrożeń
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Narządy docelowe
Respiratory system
Klasa składowania
11 - Combustible Solids
PPE (środki ochrony indywidualnej)
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Wykazy regulacyjne
Wykazy regulacyjne dotyczą głównie produktów chemicznych. Można w nich podawać ograniczoną liczbę informacji na temat produktów niechemicznych. Brak wpisu oznacza, że żaden ze składników nie znajduje się w wykazie. Użytkownik odpowiada za zagwarantowanie bezpiecznego i zgodnego z prawem stosowania produktu.
9002-07-7
CAS No.
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What is the package size of Product T6567, Trypsin from porcine pancreas?
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How can we digest protein using Product T6567, Trypsin from porcine pancreas?
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As per Sigma R and D the information is as follows: In order to efficiently digest a protein with trypsin, it must be denatured and the disulfide bonds modified by reduction and alkylation, or at least reduced. Many intact proteins are highly resistant to digestion with trypsin. If you do not want to reduce and alkylate, you can then just boil the protein with 5 mM DTT or 20 mM 2ME for 10 minutes, and then quickly cool on ice to denature the protein. This may result in a precipitate, but the trypsin will still digest the protein and it will clear within an hour or two. You can also dissolve the protein in 6 M guanidine-HCl or 8 M urea. Reduce and alkylate using the PROT-RA kit or other suitable method. Then they would have to dilute the solution to less than 2 M of either denaturant and then add the trypsin. This is the method that we use routinely in the lab.
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How can we get Trypsin sequence information for Product T6567, Trypsin from porcine pancreas?
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If you are looking for trypsin sequence information, you have to go to the NCBI Protein Data Bank.
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What can I use to solubilize this Product T6567, Trypsin from porcine pancreas?
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This product is soluble in 1mM HCL.
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Is Product T6567, Trypsin from porcine pancreas, TPCK treated?
1 answer-
The product has been treated with TPCK to remove chymotryptic activity, further purified through affinity chromatography, and lyophilized, resulting in convenient use and highly specific cleavage. This information is on the product page under application.
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