CK-RO

Creatine Kinase (CK)

from rabbit muscle

• Synonym(s): creatine phosphokinase
• UNSPSC Code: 12352204
• EC Number: 2.7.3.2 (BRENDA, IUBMB)
• NACRES: NA.54
• Biological source: rabbit muscle

Properties

• biological source: rabbit muscle
• form: lyophilized
• packaging: pkg of 100 mg (10127566001), pkg of 500 mg (10736988001)
• manufacturer/tradename: Roche
• color: white to light yellow
• optimum pH: 6.0-7.0(reverse reaction), 9.0(forward reaction)
• solubility: water: soluble
• suitability: suitable for immunoprecipitation
• NCBI accession no.: NP_001075708
• UniProt accession no.: P00563
• application(s): life science and biopharma; sample preparation
• foreign activity: ATPase <0.001%, HK <0.001%, myokinase <0.001%
• storage temp.: 2-8°C
• Gene Information: rabbit ... CKM(100009056)

Description

General description

at 25 °C (800 U/mg lyophilizate at 37 °C) with creatine phosphate and ADP as the substrates, and N-acetyl-L-cysteine as the activator.

Application

Creatine kinase has been used as a component of translation buffer used in in vitro translation assay and as a component of energy regeneration mix.

Biochem/physiol Actions

Creatine kinase (CK) is a guanidino-kinase that catalyzes the reversible phosphorylation of creatine to phosphocreatine at the expense of ATP. It plays crucial roles in rapid regeneration of ATP in cells with high demand for energy. Increased level of CK in human blood indicates diseases of the nervous system and the heart muscle, malignant hypothermia, and certain tumors. Metals such as Mg, Mn and Ca act as cofactors for CK. It also forms a substrate for protein kinase C, an important signaling molecule involved in cell growth. A product of enzymatic reaction of CK, phosphocreatine, is found to prevent HIV (human immunodeficiency virus) replication in macrophages.

Features and Benefits

Specific activity: Approximately 350U/mg lyophilizate at +25°C (800U/mg lyophilizate at +37°C) with creatine phosphate and ADP as the substrates, and N-acetyl-L-cysteine as activator.

Physical form

Lyophilizate, stabilized

Preparation Note

Storage conditions (working solution): Working solutions and storage conditions in routine applications:
Dissolve in 0.1 M imidazole buffer; aliquot and store at -15 to -25 °C.
Stable for approximately four weeks at -15 to -25 °C.
Avoid repeated freezing and thawing.

Dissolve in 0.5% NaHCO3 solution.
Do not freeze. Store protected from light.
Stable for 2 - 3 days, when stored at 2 to 8 °C.
Or
Dissolve in 30 mM glycine buffer, pH 9.0, with 0.2 mM DTT.
Stable for 2 - 3 days, when stored at 2 to 8 °C.

Analysis Note

Contaminants: <0.001% ATPase, HK, and myokinase, each

Other Notes

For life science research only. Not for use in diagnostic procedures.

One unit CK activity (+25 °C) = 2.3 U (+37 °C), creatin-P and ADP as substrates, NAcCys as reactivator.

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): does not flash
• Flash Point (°C): does not flash