A2172

Anti-α-Actin-1 (ACTA1) antibody, Mouse monoclonal

clone 5C5, purified from hybridoma cell culture

• Synonym(s): Mouse Anti-Actin
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD00164521
• Conjugate: unconjugated
• Clone: 5C5, monoclonal
• Application: ELISA (i), IHC (f), IHC (p), WB
• Citations: 215

Properties

• Product Name: Monoclonal Anti-Actin (α-Sarcomeric) antibody produced in mouse, clone 5C5, purified from hybridoma cell culture
• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: 5C5, monoclonal
• form: (Solution in 0.05M Tris, 0.5M NaCl, pH 8.0, containing 0.02% sodium azide)
• contains: 15 mM sodium azide
• species reactivity: rabbit, carp, rat, sheep, guinea pig, human, snake, bovine, frog
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using human skeletal and cardiac muscle, immunohistochemistry (frozen sections): suitable using human skeletal and cardiac muscle, indirect ELISA: suitable, western blot: suitable
• isotype: IgM
• UniProt accession no.: P68032, P68133
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ACTA1(58), ACTC1(70) ; mouse ... Rwdd2a(69519) ; rat ... Acta1(29437), Actc1(29275)

Description

General description

Monoclonal Anti-α-Sarcomeric Actin (mouse IgM isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Actin is one of the most conserved eukaryotic proteins and the isoforms show >90% overall sequence homology. It is expressed in mammals and birds as six isoforms. Four of the six represent differentiation markers of muscle tissues. The other two are found in practically all cells.

Immunogen

rabbit striated muscle actin.

Application

Monoclonal Anti-Actin (α-Sarcomeric) antibody produced in mouse has also been used

• immunofluorescence
• double immunostaining
• double labelling terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) immunofluorescence staining.

Monoclonal mouse anti-actin antibody was used as a loading control for western blot analysis of immunoprecipitated proteins from rat dorsal root ganglion cocultures.

Mouse monoclonal anti-actin (alpha-sarcomeric) antibody has been used for western blot, and immunohistochemistry applications.

Biochem/physiol Actions

Actin is a cytoskeletal protein that regulates cell motility, secretion, phagocytosis and cytokinesis. The NH2-terminal of actin may function as antigen. This terminal may also modulate actin interactions and may associate with proteins such as myosin.

Physical form

The product is provided as ascites fluid containing 0.1% sodium azide as a preservative.

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable